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Invitrogen
VCAM-1 (CD106) Polyclonal Antibody
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FIGURE: 1 / 4
VCAM-1 (CD106) Antibody (PA5-80214) in IHC (P)
Immunohistochemical analysis of VCAM1 in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-VCAM1 antibody (Product # PA5-80214) overnight at 4°C. Biotinylated goat anti-rabbit IgG was ... View More
Product Details
PA5-80214
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived rat VCAM1 recombinant protein (Position: F25-L270).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat spleen tissue, rat thymus tissue, rat testis tissue, rat kidney tissue, rat lung tissue. IHC: rat kidney tissue, rat spleen tissue, mouse spleen tissue.
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD106; DKFZp779G2333; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1; VCAM-1
Gene Aliases: CD106; Vcam-1; Vcam1; VCAM1B
UniProt ID: (Rat) P29534
Entrez Gene ID: (Rat) 25361, (Mouse) 22329
response to hypoxia
acute inflammatory response
chronic inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
heart development
aging
response to nutrient
amine metabolic process
response to zinc ion
response to ionizing radiation
viral process
membrane to membrane docking
response to lipopolysaccharide
response to nicotine
cellular response to vascular endothelial growth factor stimulus
positive regulation of T cell proliferation
response to ethanol
leukocyte tethering or rolling
oxidation-reduction process
cell chemotaxis
embryonic placenta morphogenesis
chorio-allantoic fusion
cellular response to glucose stimulus
cellular response to tumor necrosis factor
single organismal cell-cell adhesion
visceral serous pericardium development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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