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FIGURE: 1 / 3
VDAC Antibody (710606) in ICC/IF
Immunofluorescence analysis of VDAC/Porin was done on 70% confluent log phase U-87MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with VDAC/Porin Antibody (17HCLC) Recombinant Rabbit Superclonal™ (Product # 710606) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 48... View More
Product Details
710606
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
17HCLC
Immunogen
Recombinant protein corresponding to amino acids 150-250 of human Mitochondrial Porin
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Voltage-dependent anion channel (VDAC) proteins are abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. It was discovered in the mitochondrial outer membrane. It also is expressed in the plasma membrane. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apoptosis. For example, VDAC constitutes a major pathway by which metabolites such as ADP/ATP, succinate and citrate are exchanged between the cytosol and mitochondria. And VDAC1 in the plasma membrane establishes a novel level of apoptosis regulation putatively via its redox activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 1; ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 2; ATVDAC1; ATVDAC2; F2G14.210; HSR2; hVDAC1; K9I9.6; K9I9_6; MGC111064; Mitochondrial Porin; Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM; PORIN-31-HL; sperm binding protein 1a; T22N4.9; T22N4_9; unnamed protein product; VD; VDAC-1; VDAC1; VDAC2; Voltage-dependent anion-selective channel protein 1
Gene Aliases: PORIN; VDAC; VDAC-1; VDAC1
UniProt ID: (Human) P21796
Entrez Gene ID: (Human) 7416
behavioral fear response
ion transport
anion transport
lipid transport
apoptotic process
chemical synaptic transmission
neuron-neuron synaptic transmission
learning
epithelial cell differentiation
calcium ion transmembrane import into mitochondrion
positive regulation of apoptotic process
negative regulation of apoptotic process
transmembrane transport
anion transmembrane transport
negative regulation of calcium import into the mitochondrion
regulation of mitophagy
regulation of mitophagy
positive regulation of type 2 mitophagy
mitochondrial transmembrane transport
negative regulation of reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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