Proteintech

VDAC1/2 Polyclonal Antibody

42 References

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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VDAC1/2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10866-1-AP (VDAC1/Porin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

VDAC1/2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 40x lens).

VDAC1/2 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

VDAC1/2 Antibody in Immunoprecipitation (IP) — IP result of anti-VDAC1 (IP:10866-1-AP, 3ug; Detection:10866-1-AP 1:1000) with mouse skeletal muscle tissue lysate 7500ug.

Product Details

10866-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 36 publications 36 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Insect, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VDAC1/2 Fusion Protein Ag1144 (1-283 aa encoded by BC008482) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.21 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

This polyclonal antibody raised against full-length human VDAC1 protein can cross react with VDAC2.

Immunogen sequence: MAVPPTYAD LGKSARDVFT KGYGFGLIKL DLKTKSENGL EFTSSGSANT ETTKVTGSLE TKYRWTEYGL TFTEKWNTDN TLGTEITVED QLARGLKLTF DSSFSPNTGK KNAKIKTGYK REHINLGCDM DFDIAGPSIR GALVLGYEGW LAGYQMNFET AKSRVTQSNF AVGYKTDEFQ LHTNVNDGTE FGGSIYQKVN KKLETAVNLA WTAGNSNTRF GIAAKYQIDP DACFSAKVNN SSLIGLGYTQ TLKPGIKLTL SALLDGKNVN AGGHKLGLGL EFQA (1-283 aa encoded by BC008482)

Target Information

This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Mitochondrial dynamics and preconditioning in white matter.

Conditioning medicine

Bastian C,Politano S,Day J,McCray A,Brunet S,Baltan S

Mon Sep 28 00:00:00 EDT 2020

Contribution of mitochondrial ND1 3394T>C mutation to the phenotypic manifestation of Leber's hereditary optic neuropathy.

Human molecular genetics

Ji Y,Zhang J,Yu J,Wang Y,Lu Y,Liang M,Li Q,Jin X,Wei Y,Meng F,Gao Y,Cang X,Tong Y,Liu X,Zhang M,Jiang P,Zhu T,Mo JQ,Huang T,Jiang P,Guan MX

Wed May 01 00:00:00 EDT 2019

Distinctive Structural and Molecular Features of Myelinated Inhibitory Axons in Human Neocortex.

eNeuro

Micheva KD,Chang EF,Nana AL,Seeley WW,Ting JT,Cobbs C,Lein E,Smith SJ,Weinberg RJ,Madison DV

Fri Apr 19 00:00:00 EDT 2019

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

EBioMedicine

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DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Cell research

Zheng P,Chen Q,Tian X,Qian N,Chai P,Liu B,Hu J,Blackstone C,Zhu D,Teng J,Chen J

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Protective effects of mitochondrion-targeted peptide SS-31 against hind limb ischemia-reperfusion injury.

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Exogenous H₂S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.

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Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease.

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Wed Feb 28 00:00:00 EST 2018

Picroside II Exerts a Neuroprotective Effect by Inhibiting mPTP Permeability and EndoG Release after Cerebral Ischemia/Reperfusion Injury in Rats.

Journal of molecular neuroscience : MN

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Mon Jan 01 00:00:00 EST 2018

Picroside II Exerts a Neuroprotective Effect by Inhibiting mPTP Permeability and EndoG Release after Cerebral Ischemia/Reperfusion Injury in Rats.

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Li S,Wang T,Zhai L,Ge K,Zhao J,Cong W,Guo Y

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Induction of Apoptosis by the Nonstructural Protein 4 and 10 of Porcine Reproductive and Respiratory Syndrome Virus.

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Proteomics-based identification of VDAC1 as a tumor promoter in cervical carcinoma.

Oncotarget

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Bioinformatics

Protein Aliases: B36-VDAC; mVDAC1; mVDAC5; Outer mitochondrial membrane protein porin 1; Outer mitochondrial membrane protein porin 2; Plasmalemmal porin; Porin 31HL; Porin 31HM; rVDAC1; VDAC-1; VDAC-2; VDAC-5; VDAC-6; voltage-dependent anion channel 2; Voltage-dependent anion-selective channel protein 1; Voltage-dependent anion-selective channel protein 2; Voltage-dependent anion-selective channel protein 5; Voltage-dependent anion-selective channel protein 6

Gene Aliases: AL033343; mVDAC2; mVDAC6; POR; PORIN; VDAC; VDAC-1; VDAC1; VDAC2; Vdac5; Vdac6

UniProt ID: (Human) P21796, (Human) P45880, (Mouse) Q60932, (Rat) Q9Z2L0, (Mouse) Q60930, (Rat) P81155

Entrez Gene ID: (Human) 7416, (Human) 7417, (Mouse) 22333, (Rat) 83529, (Mouse) 22334, (Rat) 83531

Molecular Function: voltage-gated ion channel