Immunofluorescent analysis of Vascular Endothelial Growth Factor (VEGF, green) in Hela cells. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with (left panel) or without (right panel) a VEGF polyclonal antibody (Product # PA5-16754) at a dilution of 1:40 for at least 1 hour at room temperature, and then incubated with a Dylight 488 goat anti-rabbit IgG secondary antibody at a dilution of 1:1000 for 45 minutes at room temperature. F-actin (both panels, red) was stained by Dylight 554 Phalloidin (Product # 21834) and nuclei (both panels, blue) were stained with DAPI (Product # 46190). Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from N-terminal of human VEGF|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||3 µg/ml|
|Western Blot (WB)||1 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16754 targets Vascular Endothelial Growth Factor (VEGF) in IF, IHC (P), IP, and WB applications and shows reactivity with Human samples.
The PA5-16754 immunogen is a synthetic peptide derived from N-terminal of human VEGF.
VEGF belongs to a family of heparin binding growth factors and is the major mediator of vasculogenesis and angiogenesis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. VEGF is a 45 kDa homodimeric glycoprotein, but other isoforms can be produced through alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Deletion of IL-33R attenuates VEGF expression and enhances necrosis in mammary carcinoma.
PA5-16754 was used in immunohistochemistry - paraffin section to study the attenuation of VEGF expression and enhancement of necrosis in mammary carcinoma by deletion of IL-33R
|Milosavljevic MZ,Jovanovic IP,Pejnovic NN,Mitrovic SL,Arsenijevic NN,Simovic Markovic BJ,Lukic ML||Oncotarget (7:18106)||2016|
Endocardial-to-mesenchymal transformation and mesenchymal cell colonization at the onset of human cardiac valve development.
PA5-16754 was used in immunohistochemistry - paraffin section to characterize human cardiac valve development by endocardial-to-mesenchymal transformation and mesenchymal cell colonization
|Monaghan MG,Linneweh M,Liebscher S,Van Handel B,Layland SL,Schenke-Layland K||Development (Cambridge, England) (143:473)||2016|
Combined use of COX-1 and VEGF immunohistochemistry refines the histopathologic prognosis of renal cell carcinoma.
PA5-16754 was used in immunohistochemistry - paraffin section to characterize the histopathologic prognosis of renal cell carcinoma by combined use of EGF and COX-1 immunohistochemistry
|Osman WM,Youssef NS||International journal of clinical and experimental pathology (8:8165)||2015|
Tumor endothelium FasL establishes a selective immune barrier promoting tolerance in tumors.
PA5-16754 was used in immunohistochemistry to study the rmechanism by which tumor endothelial FasL promotes tumor immune tolerance
|Motz GT,Santoro SP,Wang LP,Garrabrant T,Lastra RR,Hagemann IS,Lal P,Feldman MD,Benencia F,Coukos G||Nature medicine (20:607)||2014|
Aging-related changes of optic nerve of Wistar albino rats.
PA5-16754 was used in immunohistochemistry to study age-related deterioration of the optic nerve in a rat model
|El-Sayyad HI,Khalifa SA,El-Sayyad FI,Al-Gebaly AS,El-Mansy AA,Mohammed EA||Age (Dordrecht, Netherlands) (36:519)||2014|
Proliferation and maturation of microvessels in arteriovenous malformations--expression patterns of angiogenic and cell cycle-dependent factors.
PA5-16754 was used in immunohistochemistry to study the patterns of proliferation and maturation of microvessels in arteriovenous malformations
|Meijer-Jorna LB,van der Loos CM,Teeling P,de Boer OJ,Florquin S,van der Horst CM,van der Wal AC||Journal of cutaneous pathology (39:610)||2012|
Castration resistant prostate cancer is associated with increased blood vessel stabilization and elevated levels of VEGF and Ang-2.
PA5-16754 was used in immunohistochemistry to study blood vessel stabilization and the expression of pro-angiogenic VEGF and Ang-2 in castration-resistant prostate cancer
|Tomi¿ TT,Gustavsson H,Wang W,Jennbacken K,Welén K,Damber JE||The Prostate (72:705)||2012|
Profilin-1 phosphorylation directs angiocrine expression and glioblastoma progression through HIF-1¿ accumulation.
PA5-16754 was used in western blot to study the roles of angiocrine secretion and accumulation of HIF-1alpha in the mechanism by which phosphorylation of endothelial cell profilin-1 promotes the progression of glioblastoma
|Fan Y,Potdar AA,Gong Y,Eswarappa SM,Donnola S,Lathia JD,Hambardzumyan D,Rich JN,Fox PL||Nature cell biology (16:445)||2014|
Inflammation modulates expression of laminin in the central nervous system following ischemic injury.
PA5-16754 was used in western blot to study the role of COX-2 in the upregulation of CNS laminin in a murine model of ischemic injury
|Ji K,Tsirka SE||Journal of neuroinflammation (9:null)||2012|