Invitrogen

VEGF Receptor 2 Polyclonal Antibody

View all (59) FLK1 (VEGF Receptor 2) antibodies

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Product Details

PA5-143790

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide (coupled to carrier protein) corresponding to amino acids from the N-terminal region of human VEGFR-2. This sequence has high homology to rat and mouse VEGFR-2, and no significant homology to other VEGFR family members.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2943018

Product Specific Information

The antibody detects a 180 kDa* band corresponding to VEGFR-2 in western blots of human endothelial cells, and shows strong reactivity toward recombinant human VEGFR-2, but not VEGFR-1 or VEGFR-3. The antibody also works for immunofluorescent staining of VEGFR-2 in HUVEC cells.

Target Information

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD309; CD309 antigen; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; FLK1 kinase insert domain receptor (a type III receptor tyrosine kinase) (VEGF receptor 2); FLK1 kinase insert domain receptor (VEGF receptor 2); KDR; kinase insert domain protein receptor; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; soluble VEGFR2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2

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Gene Aliases: 6130401C07; CD309; Flk-1; FLK1; KDR; Krd-1; Ly73; sVEGFR-2; VEGFR; VEGFR-2; VEGFR2

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UniProt ID: (Human) P35968, (Rat) O08775, (Mouse) P35918

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Entrez Gene ID: (Human) 3791, (Rat) 25589, (Mouse) 16542

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Function(s)
protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity integrin binding protein binding ATP binding growth factor binding Hsp90 protein binding vascular endothelial growth factor binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor
Process(es)
angiogenesis vasculogenesis positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis endothelium development transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cell proliferation regulation of cell shape positive regulation of endothelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling viral process positive regulation of macroautophagy peptidyl-tyrosine phosphorylation signal transduction by protein phosphorylation extracellular matrix organization positive regulation of cell migration embryonic hemopoiesis calcium-mediated signaling using intracellular calcium source cellular response to vascular endothelial growth factor stimulus positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway peptidyl-tyrosine autophosphorylation negative regulation of apoptotic process positive regulation of MAPK cascade positive regulation of angiogenesis protein autophosphorylation vascular endothelial growth factor receptor signaling pathway positive regulation of positive chemotaxis positive regulation of nitric-oxide synthase biosynthetic process positive regulation of focal adhesion assembly positive regulation of mitochondrial depolarization positive regulation of ERK1 and ERK2 cascade positive regulation of mitochondrial fission negative regulation of endothelial cell apoptotic process positive regulation of vasculogenesis ovarian follicle development response to hypoxia lymph vessel development positive regulation of mesenchymal cell proliferation endocardium development endochondral bone growth positive regulation of cytosolic calcium ion concentration aging male gonad development cell migration hemopoiesis hyaluronan metabolic process lung development positive regulation of BMP signaling pathway positive regulation of TOR signaling vascular endothelial growth factor signaling pathway response to drug surfactant homeostasis negative regulation of neuron apoptotic process cell fate commitment endothelial cell differentiation regulation of endothelial cell differentiation positive regulation of vasodilation positive regulation of long-term neuronal synaptic plasticity lung alveolus development cell maturation branching morphogenesis of an epithelial tube neuron projection morphogenesis positive regulation of epithelial cell proliferation positive regulation of neurogenesis positive regulation of calcium-mediated signaling calcium ion homeostasis response to oxygen levels regulation of hematopoietic progenitor cell differentiation regulation of endothelial cell proliferation protein phosphorylation multicellular organism development phosphorylation cell differentiation positive regulation of vascular endothelial growth factor receptor signaling pathway blood vessel endothelial cell differentiation
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