Immunohistochemistry analysis of FLT-4 / VEGFR3 showing staining in the cytoplasm of paraffin-embedded human placenta tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- FLT-4 / VEGFR3 Polyclonal Antibody (360200) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Raised against the C-term of human VEGF Receptor-3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.01% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression of factors involved in the regulation of angiogenesis in the full-term human placenta: Effects of in vitro fertilization.
36-0200 was used in western blot to assess regulation of angiogenesis in the full-term human placenta and the expression of factors involved that effect in vitro fertilization
|Li C,Zhang Y,Tang L,Zhao H,Gao C,Gao L,Cui Y,Liu J||Reproductive biology (16:104)||2016|
|Human||Not Cited||Immunohistochemical expression and distribution of VEGFR-3 in malignant mesothelioma.||Filho AL,Baltazar F,Bedrossian C,Michael C,Schmitt FC||Diagnostic cytopathology (35:786)||2007|