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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:200 | - |
Immunohistochemistry (Frozen) (IHC (F)) |
1:600 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Guinea pig / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Peptide corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (Cytoplasmic, N-terminus) |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Concentration |
0.8 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.4, with 1% BSA |
Contains |
0.05% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2857241 |
Reconstitute antibody with 50 µL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Peptide sequence is: (C)GEFGGHD KPKITAWE
Homology: Mouse, human amino acid residues are identical.
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GABA and glycine transporter; GVAT; hVIAAT; mVGAT; mVIAAT; rGVAT; solute carrier family 32 (GABA vesicular transporter), member 1; Solute carrier family 32 member 1; vasicular inhibitory amino acid transporter 10D; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter
Gene Aliases: R75019; SLC32A1; VGAT; VIAAT
UniProt ID: (Human) Q9H598, (Rat) O35458, (Mouse) O35633
Entrez Gene ID: (Human) 140679, (Rat) 83612, (Mouse) 22348
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