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Invitrogen
VGAT Polyclonal Antibody
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FIGURE: 1 / 2
VGAT Antibody (PA5-96231) in WB
Western blot analysis of VGAT in extracts of various cell lines. Samples were incubated with VGAT Polyclonal antibody (Product # PA5-96231) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-96231
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-525 of human SLC32A1 (Q9H598)
Conjugate
Form
Liquid
Concentration
2.79 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse spinal cord, Mouse brain; Cellular Location: Cytoplasmic vesicle membrane, Multi-pass membrane protein
Immunogen sequence: MATLLRSKLS NVATSVSNKS QAKMSGMFAR MGFQAATDEE AVGFAHCDDL DFEHRQGLQM DILKAEGEPC GDEGAEAPVE GDIHYQRGSG APLPPSGSKD QVGGGGEFGG HDKPKITAWE AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF LIIFAAVVCC YTGKILIACL YEENEDGEVV RVRDSYVAIA NACCAPRFPT LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ KSWSIIATAV LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL KGLFALVAYL TWADETKEVI TDNLPGSIRA VVNIFLVAKA LLSYPLPFFA AVEVLEKSLF QEGSRAFFPA CYSGDGRLKS WGLTLRCALV VFTLLMAIYV PHFALLMGLT GSLTGAGLCF LLPSLFHLRL LWRKLLWHQV FFDVAIFVIG GICSVSGFVH SLEGLIEAYR TNAED
Target Information
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GABA and glycine transporter; GVAT; hVIAAT; mVGAT; mVIAAT; rat UNC-47 homolog; rGVAT; RUNC-47; solute carrier family 32 (GABA vesicular transporter), member 1; Solute carrier family 32 member 1; vasicular inhibitory amino acid transporter 10D; Vesicular GABA and glycine transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter
Gene Aliases: R75019; SLC32A1; VGAT; VIAAT
UniProt ID: (Human) Q9H598, (Rat) O35458, (Mouse) O35633
Entrez Gene ID: (Human) 140679, (Rat) 83612, (Mouse) 22348
amino acid transmembrane transport
neurotransmitter transport
aging
gamma-aminobutyric acid transport
glycine transport
proton transport
sodium ion transmembrane transport
L-alpha-amino acid transmembrane transport
transport
ion transport
neurotransmitter secretion
amine transport
hippocampus development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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