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Invitrogen
VGLL2 Polyclonal Antibody
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FIGURE: 1 / 2
VGLL2 Antibody (PA5-100693) in ICC/IF
Immunocytochemistry analysis of VGLL2 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with VGLL2 polyclonal antibody (Product # PA5-100693) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100693
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human VGLL2(Accession Q8N8G2), corresponding to amino acid residues S116-H166.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total VGLL2.
Target Information
VGLL2 is the master regulator in the wing development in Drosophila, which acts through a complex by interacting with scalloped, a homolog of vertebrate TEF proteins. Northern and dot blot analyses of human adult tissues detected VGLL2 expression in skeletal muscle. In situ hybridization revealed VGLL2 expression in differentiating somites and branchial arches during mouse embryogenesis. VITO1 is the clone of VGLL2. It contains a scalloped interaction domain (SID). VITO1 is more highly expressed in differentiated myotubes from the mouse C2C12 myogenic cell line than in undifferentiated cells. During differentiation in mouse C2C12 cell line, VGLL2 mRNA levels increase and VGLL2 protein translocates from the cytoplasm to the nucleus. Interference with VITO1 expression by small interfering RNA attenuated differentiation in C2C12 muscle cells. Therefore, this protein is an important component of the myogenic program.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein VITO1; RP1-179P9.2; Transcription cofactor vestigial-like protein 2; unnamed protein product; Vestigial and Tondu related protein 1; vestigial like 2; vestigial like 2 homolog; Vgl-2
Gene Aliases: C130057C21Rik; vgl-2; VGL2; VGLL2; VITO-1; VITO1
UniProt ID: (Human) Q8N8G2, (Mouse) Q8BWB4
Entrez Gene ID: (Human) 245806, (Mouse) 215031, (Rat) 309772
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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