Immunofluorescence analysis of VGLUT1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VGLUT1 Rabbit Polyclonal Antibody (482400) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the internal region of the mouse VGLUT1 protein (Accession# NP_892038, Q7TQH3), which is 93% homologous to human and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (IHC)||2-3 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
48-2400 has been successfully used in immunofluorescence analysis of vGlut1 in human glutamate neurons derived from iPSCs.
A Na(+)-dependent inorganic phosphate cotransporter; may be involved in neuronal Na+ transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Auditory nerve synapses persist in ventral cochlear nucleus long after loss of acoustic input in mice with early-onset progressive hearing loss.
48-2400 was used in immunohistochemistry - free floating to report the gross morphology of the ventral cochlear nucleus and auditory nerve synapse integrity in mice with early-onset progressive sensorineural hearing loss
|McGuire B,Fiorillo B,Ryugo DK,Lauer AM||Brain research (1605:22)||2015|
Directing human induced pluripotent stem cells into a neurosensory lineage for auditory neuron replacement.
48-2400 was used in immunocytochemistry to study how to differentiate hiPSCs into a neurosensory lineage.
|Gunewardene N,Bergen NV,Crombie D,Needham K,Dottori M,Nayagam BA||BioResearch open access (3:162)||2014|
Genetic removal of matrix metalloproteinase 9 rescues the symptoms of fragile X syndrome in a mouse model.
48-2400 was used in immunocytochemistry to investigate the involvement of matrix metalloproteinase 9 in the phenotype of fragile X syndrome.
|Sidhu H,Dansie LE,Hickmott PW,Ethell DW,Ethell IM||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:9867)||2014|