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Immunofluorescence analysis of vGlut1 (red) in human glutamate neurons derived from iPSCs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.2% Triton X-100 in PBS for 10 minutes, and blocked with 5% donkey serum in PBS for 15 minutes at room temperature. Cells were stained with a vGlut1 rabbit polyclonal antibody (Product # 48-2400) diluted at 1:1000 in 5% donkey serum overnight at 4°C, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 546 conjugate (Product # A11010) at a dilution of 1:1000 for 1 hour at room temperature (green). Note: Data courtesy of Innovators Program.
|Tested species reactivity||Human , Rat , Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the internal region of the mouse VGLUT1 protein (Accession# NP_892038, Q7TQH3), which is 93% homologous to human and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||2-3 ug/ml|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
48-2400 has been successfully used in immunofluorescence analysis of vGlut1 in human glutamate neurons derived from iPSCs.
A Na(+)-dependent inorganic phosphate cotransporter; may be involved in neuronal Na+ transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Genetic removal of matrix metalloproteinase 9 rescues the symptoms of fragile X syndrome in a mouse model.
48-2400 was used in immunocytochemistry to investigate the involvement of matrix metalloproteinase 9 in the phenotype of fragile X syndrome.
|Sidhu H,Dansie LE,Hickmott PW,Ethell DW,Ethell IM||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:9867)||2014|
BNPI, 2900052E22Rik, VGLUT1, Vglut1, AI851913
brain-specific Na(+)-dependent inorganic phosphate cotransporter, brain-specific Na-dependent inorganic phosphate cotransporter, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7, solute carrier family 17 member 7, vesicular glutamate transporter 1, Vesicular glutamate transporter 1, VGlU1, Bnpi