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Invitrogen
VGLUT1 Polyclonal Antibody
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FIGURE: 1 / 24
VGLUT1 Antibody (48-2400) in ICC/IF
Immunofluorescence analysis of VGLUT1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VGLUT1 Rabbit Polyclonal Antibody (Product # 48-2400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product... View More
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.jpg?time=20220826?time=20220826 "VGLUT1 Antibody (48-2400) in ICC/IF")
Product Details
48-2400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the internal region of the mouse VGLUT1 protein (Accession# NP_892038, Q3TXX4), which is 93% homologous to human and rat.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific for the VGLUT1 protein. On western blots, it identifies the target band at ~55 kDa. Reactivity has been confirmed with mouse brain homogenates by western blotting, with mouse hippocampus frozen sections by immunohistochemistry, and in immunofluorescence analysis with human glutamate neurons derived from iPSCs.
Target Information
A Na(+)-dependent inorganic phosphate cotransporter; may be involved in neuronal Na+ transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Brain-specific Na(+)-dependent inorganic phosphate cotransporter; brain-specific Na-dependent inorganic phosphate cotransporter; SLC17A1; SLC17A1/VGLUT1; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; solute carrier family 17 (vesicular glutamate transporter), member 7; Solute carrier family 17 member 7; Vesicular glutamate transporter 1; VGlU1; VGLUT 1; VGluT1
Gene Aliases: 2900052E22Rik; AI851913; BNPI; SLC17A7; VGLUT1
UniProt ID: (Human) Q9P2U7, (Rat) Q62634, (Mouse) Q3TXX4
Entrez Gene ID: (Human) 57030, (Rat) 116638, (Mouse) 72961
L-glutamate transmembrane transporter activity
inorganic phosphate transmembrane transporter activity
extracellular-glutamate-gated chloride channel activity
sodium:inorganic phosphate symporter activity
sodium-dependent phosphate transmembrane transporter activity
symporter activity
secondary carrier transporter
neural retina development
ion transport
phosphate ion transport
neurotransmitter transport
brain development
long-term memory
glutamate secretion
synaptic transmission, glutamatergic
phosphate ion transmembrane transport
sodium ion transmembrane transport
sequestering of neurotransmitter
sodium-dependent phosphate transport
L-glutamate import
excitatory postsynaptic potential
L-glutamate transmembrane transport
synaptic vesicle lumen acidification
chloride transmembrane transport
chemical synaptic transmission
transport
sodium ion transport
response to stimulus
transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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