Invitrogen

VGLUT1 Polyclonal Antibody

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VGLUT1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of VGLUT1 in 4% paraformaldehyde-fixed rat E18 primary cortical neurons using VGLUT1 polyclonal antibody (Product # PA5-85764) at a dilution of 1:500. Sample was then incubated with beta Tubulin 3/ TUJ1 (red) and DAPI secondary antibody at a dilution of 1:500.

VGLUT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of VGLUT1 in paraffin-embedded mouse brain using VGLUT1 polyclonal antibody (Product # PA5-85764) at a dilution of 1:500.

VGLUT1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry analysis of VGLUT1 in frozen adult mouse cerebellum using VGLUT1 polyclonal antibody (Product # PA5-85764) at a dilution of 1:250. Sample was then incubated with beta Tubulin 3/ TUJ1 (red) and DAPI secondary antibody at a dilution of 1:500.

VGLUT1 Antibody in Western Blot (WB) — Western blot analysis of VGLUT1 in various tissue extracts using VGLUT1 polyclonal antibody (Product # PA5-85764) using 50 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse VGluT1.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2792901

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Human (96%), Mouse (100%), Rat (98%), Bovine (100%).

Positive Control: mouse brain, rat brain.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

A Na(+)-dependent inorganic phosphate cotransporter; may be involved in neuronal Na+ transport.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Brain-specific Na(+)-dependent inorganic phosphate cotransporter; brain-specific Na-dependent inorganic phosphate cotransporter; SLC17A1; SLC17A1/VGLUT1; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; Solute carrier family 17 member 7; Vesicular glutamate transporter 1; VGlU1; VGLUT 1; VGluT1

Gene Aliases: 2900052E22Rik; AI851913; BNPI; Slc17a7; Vglut1

UniProt ID: (Rat) Q62634, (Mouse) Q3TXX4

Entrez Gene ID: (Rat) 116638, (Mouse) 72961

Molecular Function: secondary carrier transporter