Invitrogen

VGLUT1 Polyclonal Antibody

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VGLUT1 Antibody in Immunocytochemistry (ICC/IF) — VGLUT1 Polyclonal Antibody detects VGluT1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGluT1 stained by VGLUT1 Polyclonal Antibody (Product # PA5-85764) diluted at 1:500. Red: Tau, stained by Phospho-Tau (Ser262) Polyclonal Antibody [GT287]diluted at 1:500. Blue: Fluoroshield with DAPI .

VGLUT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — VGLUT1 Polyclonal Antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGluT1 stained by VGLUT1 Polyclonal Antibody (Product # PA5-85764) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

VGLUT1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — VGLUT1 Polyclonal Antibody detects VGluT1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: VGluT1 protein stained by VGLUT1 Polyclonal Antibody (Product # PA5-85764) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .

VGLUT1 Antibody in Western Blot (WB) — Western Blot using VGLUT1 Polyclonal Antibody (Product # PA5-85764). Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGLUT1 Polyclonal Antibody (Product # PA5-85764) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Product Details

PA5-85764

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the C-terminus region of mouse VGluT1. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.4 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 1% BSA, 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2792901

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Human (96%), Mouse (100%), Rat (98%), Bovine (100%).

Positive Control: mouse brain, rat brain.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

A Na(+)-dependent inorganic phosphate cotransporter; may be involved in neuronal Na+ transport.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Brain-specific Na(+)-dependent inorganic phosphate cotransporter; brain-specific Na-dependent inorganic phosphate cotransporter; SLC17A1; SLC17A1/VGLUT1; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; Solute carrier family 17 member 7; Vesicular glutamate transporter 1; VGlU1; VGLUT 1; VGluT1

Gene Aliases: 2900052E22Rik; AI851913; BNPI; Slc17a7; Vglut1

UniProt ID: (Rat) Q62634, (Mouse) Q3TXX4

Entrez Gene ID: (Rat) 116638, (Mouse) 72961

Molecular Function: secondary carrier transporter