Invitrogen

VHL Polyclonal Antibody

Name: VHL Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-27322

Advanced Verification

3 Published Figures

7 References

View all (20) VHL antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

VHL Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

VHL Antibody (PA5-27322) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of VHL was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: VHL Polyclonal Antibody (Product # PA5-27322) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

VHL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VHL Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

VHL Antibody in Immunoprecipitation (IP)

Product Image Gallery

VHL Antibody — Figure 3. UBE2C mediates shear-dependent expression of pVHL and HIF-1&agr; in human aortic valve endothelial cells (HAVECs), and UBE2C, pVHL, and HIF1&agr; are expressed in a side-dependent manner in human aortic valve (AV) leaflets. A , Depicts a hypothesis that overexpression of UBE2C ubiquitinates pVHL, leading to increased HIF-1&agr; level, endothelial inflammation, and endothelial-mesenchymal-transition (EndMT). B , HAVECs sheared for 72 h by stable flow (SF) or disturbed flow (DF) were immunostained with antibodies for pVHL or HIF-1&agr; ( B ) and quantification of the image was done using ImageJ ( C ), or by qPCR ( D ) normalized to 18S (n=3-4). E , HAVECs treated with siCtrl or siUBE2C for 24 h, followed by exposure to DF for 24 h, were analyzed by Western blot ( E ) and Image J quantification ( F ) for pVHL (n=5). For the HIF-1&agr; study, HAVECs exposed to DF for 72 h were immunostained with HIF-1&agr; ( G ) and Image J quantified ( H ; n=6). I , Human AVs with sclerosis were stained with antibodies to UBE2C, pVHL, HIF-1&agr;, Twist1, and Runx2 (runt-related transcription factor 2) with DAPI nuclear staining and ( J ) show quantification of the fluorescent intensities of each staining in the endothelial layer on each side using Image J (n=5-8). Mean +- SEM, * P <0.05. Fib indicates fibrosa; and Ven, ventricularis. {TM}

Product Details

PA5-27322

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Affinity Purification (AP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

C. elegans, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Von Hippel Lindau. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2544798

Product Specific Information

Recommended positive controls: Myc-DDK-tagged VHL-transfected 293T.

Predicted reactivity: Mouse (93%), Rat (91%), Dog (95%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: elongin binding protein; Protein G7; pVHL; VHL19; VHLp18(MEA); Von Hippel Lindau; von hippel-lindau; von Hippel-Lindau disease tumor suppressor; von Hippel-Lindau syndrome homolog; von Hippel-Lindau syndrome protein homolog; von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase

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Gene Aliases: HRCA1; pVHL; RCA1; VHL; VHL1; Vhlh

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UniProt ID: (Human) P40337, (Mouse) P40338

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Entrez Gene ID: (Human) 7428, (Mouse) 22346

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Function(s)

ubiquitin-protein transferase activity protein binding transcription factor binding enzyme binding ubiquitin protein ligase activity protein complex binding ubiquitin-protein ligase

Process(es)

negative regulation of transcription from RNA polymerase II promoter cell morphogenesis regulation of transcription, DNA-templated proteolysis negative regulation of cell proliferation protein ubiquitination negative regulation of apoptotic process positive regulation of cell differentiation positive regulation of transcription, DNA-templated protein stabilization regulation of transcription from RNA polymerase II promoter in response to hypoxia negative regulation of transcription from RNA polymerase II promoter in response to hypoxia angiogenesis response to hypoxia type B pancreatic cell differentiation pancreatic A cell differentiation NLS-bearing protein import into nucleus proteasomal protein catabolic process protein transport protein catabolic process neuron differentiation extracellular matrix organization regulation of catecholamine metabolic process blood vessel endothelial cell migration negative regulation of endothelial cell differentiation protein heterooligomerization regulation of thymocyte apoptotic process negative regulation of thymocyte apoptotic process negative regulation of hypoxia-inducible factor-1alpha signaling pathway regulation of apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VHL Polyclonal Antibody

VHL Antibody (PA5-27322) in ICC/IF

Product Details

PA5-27322

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Affinity Purification (AP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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