Invitrogen

VIP Polyclonal Antibody

1 Published Figure

6 References

View all (34) VIP antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (1)

VIP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

VIP Antibody (PA5-78224) in IHC (P)

VIP Polyclonal Antibody detects VIP protein at cytosol on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. VIP Polyclonal Antibody (Product # PA5-78224) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

VIP Antibody in Immunohistochemistry (Frozen) (IHC (F))

VIP Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-78224

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VIP. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2736784

Product Specific Information

Positive Control: SK-N-SH , SK-N-AS, PC-3

Predicted Reactivity: Pig (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

VIP encodes a protein that belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tiered sympathetic control of cardiac function revealed by viral tracing and single cell transcriptome profiling.

eLife

Sharma S,Littman R,Tompkins JD,Arneson D,Contreras J,Dajani AH,Ang K,Tsanhani A,Sun X,Jay PY,Herzog H,Yang X,Ajijola OA

PA5-78224 was used in Immunohistochemistry to target specific neuronal subtypes for sympathetic blockade in cardiac disease.

Wed May 10 00:00:00 UTC 2023

Activation of the anterior cingulate cortex ameliorates anxiety in a preclinical model of fetal alcohol spectrum disorders.

Translational psychiatry

Hwang HM,Hashimoto-Torii K

PA5-78224 was used in Immunohistochemistry to show that reduced excitation in the anterior cingulate cortex (ACC) is a cause of anxiety in the prenatal alcohol exposure mice, providing critical insights into the ACC-basolateral amygdala (BLA) neural circuit as a potential target for treating anxiety in fetal alcohol spectrum disorders patients.

Thu Jan 20 00:00:00 UTC 2022

Activation of the anterior cingulate cortex ameliorates anxiety in a preclinical model of fetal alcohol spectrum disorders.

Translational psychiatry

Hwang HM,Hashimoto-Torii K

Thu Jan 20 00:00:00 UTC 2022

Identification of TPBG-Expressing Amacrine Cells in DAT-tdTomato Mouse.

Investigative ophthalmology & visual science

Huang W,Xu Q,Liu F,Su J,Xiao D,Tang L,Hao ZZ,Liu R,Xiang K,Bi Y,Miao Z,Liu X,Liu Y,Liu S

PA5-78224 was used in Immunohistochemistry to identify trophoblast glycoprotein (TPBG)-expressing GABAergic amacrine cells (ACs) that were selectively labeled in DAT-tdTomato transgenic mice.

Mon May 02 00:00:00 UTC 2022

Loss of ARHGAP15 affects the directional control of migrating interneurons in the embryonic cortex and increases susceptibility to epilepsy.

Frontiers in cell and developmental biology

Liaci C,Camera M,Zamboni V,Sarò G,Ammoni A,Parmigiani E,Ponzoni L,Hidisoglu E,Chiantia G,Marcantoni A,Giustetto M,Tomagra G,Carabelli V,Torelli F,Sala M,Yanagawa Y,Obata K,Hirsch E,Merlo GR

PA5-78224 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to indicate that ARHGAP15 imposes a fine negative regulation on RAC1 that is required for morphological maturation and directional control during CIN migration, with consequences on their laminar distribution and inhibitory function.

Tue Jan 03 00:00:00 UTC 2023

VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD).

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society

Semaniakou A,Chappe F,Anini Y,Chappe V

PA5-78224 was used in Immunohistochemistry to investigate effects of vasoactive intestinal peptide on cystic fibrosis related diabetes in mice.

Wed Sep 01 00:00:00 UTC 2021

Deletion of AMPA receptor GluA1 subunit gene (Gria1) causes circadian rhythm disruption and aberrant responses to environmental cues.

Translational psychiatry

Ang G,Brown LA,Tam SKE,Davies KE,Foster RG,Harrison PJ,Sprengel R,Vyazovskiy VV,Oliver PL,Bannerman DM,Peirson SN

Published figure using VIP polyclonal antibody (Product # PA5-78224) in Immunocytochemistry

Mon Nov 15 00:00:00 UTC 2021

Deletion of AMPA receptor GluA1 subunit gene (Gria1) causes circadian rhythm disruption and aberrant responses to environmental cues.

Translational psychiatry

Ang G,Brown LA,Tam SKE,Davies KE,Foster RG,Harrison PJ,Sprengel R,Vyazovskiy VV,Oliver PL,Bannerman DM,Peirson SN

Published figure using VIP polyclonal antibody (Product # PA5-78224) in Immunocytochemistry

Mon Nov 15 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: MGC13587; PACAP-R-2; PHI, peptide histidine isoleucine; prepro-VIP; vasoactive intestinal polypeptide; VIP peptides; VIP-R-1

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Gene Aliases: PHM27; VIP; vip/phi27

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UniProt ID: (Human) P01282, (Rat) P01283, (Mouse) P32648

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Entrez Gene ID: (Human) 7432, (Rat) 117064, (Mouse) 22353

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Function(s)

hormone activity neuropeptide hormone activity protein binding receptor binding neuropeptide

Process(es)

positive regulation of endothelial cell proliferation G-protein coupled receptor signaling pathway body fluid secretion learning or memory positive regulation of cell proliferation positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway regulation of protein localization negative regulation of apoptotic process negative regulation of potassium ion transport positive regulation of protein catabolic process positive regulation of vasodilation mRNA stabilization negative regulation of smooth muscle cell proliferation regulation of sensory perception of pain positive regulation of penile erection prolactin secretion regulation of signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VIP Polyclonal Antibody

VIP Antibody (PA5-78224) in IHC (P)

Product Details

PA5-78224

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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