Invitrogen

VIP Polyclonal Antibody

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VIP Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of VIP was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: VIP Polyclonal Antibody (Product # PA5-78224) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

VIP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — VIP Polyclonal Antibody detects VIP protein at cytosol on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. VIP Polyclonal Antibody (Product # PA5-78224) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

VIP Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry (Frozen) analysis of VIP was performed in frozen-sectioned mouse thalamus tissue using VIP Polyclonal Antibody (Product # PA5-78224) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.

VIP Antibody in Western Blot (WB) — Western Blot using VIP Polyclonal Antibody (Product # PA5-78224). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with VIP Polyclonal Antibody (Product # PA5-78224) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

VIP Antibody in Immunohistochemistry (IHC) — Fig. 4 Immunofluorescence cFos, VIP, and AVP signals. a A schematic depicting the timeline of the experiment: For half of the animals ( n = 3 wild-type and three GluA1 KO mice), no light pulse was given prior to perfusion and they were perfused at ~ZT15.5 (day 1, LP -); the remaining animals perfused on day 2 ( n = 3 wild-type and three GluA1 KO mice) received a nocturnal light pulse ( LP +) which started at ZT14. Star symbols indicate the Zeitgeber time of perfusion, which was ~90 min after the onset of the nocturnal light (~ZT15.5). Thus, the ZTs at which perfusion was performed were matched between LP - and LP + conditions. b Representative images of immunofluorescence cFos, VIP, and AVP staining in the SCN (scale bar = 100 mum). In the middle multi-panels, brighter colours indicate higher immunofluorescence ( F ) intensity, whereas darker colours indicate lower F intensity. A schematic delineating the core (ventromedial) vs. shell (dorsolateral) subregions of the SCN is shown on the right (D dorsal, V ventral, M medial, and L lateral). c Mean F intensities (+-standard errors of the mean) from all three channels across the entire mediolateral extent of the SCN. Nocturnal-light-induced cFos signals were attenuated in GluA1 knockouts (*two-way ANOVA for LP + conditions, Genotype x Mediolateral Extent interaction p = 0.019). VIP and AVP data were pooled across LP - and + LP ( n = 6 per genotype). The vertical dashed lines represent the location of the midline, and the 60-mum

Product Details

PA5-78224

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VIP. The exact sequence is proprietary. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.22 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol, 1% BSA
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2736784

Product Specific Information

Positive Control: SK-N-SH , SK-N-AS, PC-3

Predicted Reactivity: Pig (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

VIP encodes a protein that belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Activation of the anterior cingulate cortex ameliorates anxiety in a preclinical model of fetal alcohol spectrum disorders.

Translational psychiatry

Hwang HM,Hashimoto-Torii K

PA5-78224 was used in Immunohistochemistry to show that reduced excitation in the anterior cingulate cortex (ACC) is a cause of anxiety in the prenatal alcohol exposure mice, providing critical insights into the ACC-basolateral amygdala (BLA) neural circuit as a potential target for treating anxiety in fetal alcohol spectrum disorders patients.

Thu Jan 20 00:00:00 EST 2022

Deletion of AMPA receptor GluA1 subunit gene (Gria1) causes circadian rhythm disruption and aberrant responses to environmental cues.

Translational psychiatry

Ang G,Brown LA,Tam SKE,Davies KE,Foster RG,Harrison PJ,Sprengel R,Vyazovskiy VV,Oliver PL,Bannerman DM,Peirson SN

PA5-78224 was used in Immunohistochemistry to investigate the role of GluA1 in circadian and sleep behaviour.

Mon Nov 15 00:00:00 EST 2021

Deletion of AMPA receptor GluA1 subunit gene (Gria1) causes circadian rhythm disruption and aberrant responses to environmental cues.

Translational psychiatry

Ang G,Brown LA,Tam SKE,Davies KE,Foster RG,Harrison PJ,Sprengel R,Vyazovskiy VV,Oliver PL,Bannerman DM,Peirson SN

PA5-78224 was used in Immunohistochemistry to investigate the role of GluA1 in circadian and sleep behaviour.

Mon Nov 15 00:00:00 EST 2021

VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD).

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society

Semaniakou A,Chappe F,Anini Y,Chappe V

PA5-78224 was used in Immunohistochemistry to investigate effects of vasoactive intestinal peptide on cystic fibrosis related diabetes in mice.

Wed Sep 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Intestinal peptide PHI-27; Intestinal peptide PHI-42; Intestinal peptide PHM-27; Intestinal peptide PHV-42; MGC13587; PACAP-R-2; Peptide histidine isoleucinamide 27; Peptide histidine methioninamide 27; Peptide histidine valine 42; PHI, peptide histidine isoleucine; prepro-VIP; Vasoactive intestinal peptide; Vasoactive intestinal polypeptide; VIP; VIP peptides

Gene Aliases: PHM27; VIP; vip/phi27

UniProt ID: (Human) P01282, (Rat) P01283, (Mouse) P32648

Entrez Gene ID: (Human) 7432, (Rat) 117064, (Mouse) 22353

Molecular Function: neuropeptide