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Invitrogen
VMP1 Polyclonal Antibody
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FIGURE: 1 / 2
VMP1 Antibody (PA5-77784) in ICC/IF
Immunofluorescent analysis of VMP1 in human colon carcinoma cell line (RKO). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with VMP1 polyclonal antibody (Product # PA5-77784) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Rabbit 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: A) DAPI nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) VMP1 Antibody, D) Merged image. Magnification: 60x.
Product Details
PA5-77784
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide from the C-terminal of Human VMP1 (aa 391-402)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:1,000 dilution of PA5-77784 was sufficient for detection of VMP1 in 15 µg of Human 293T Cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 46 kDa.
Target Information
VMP1 is a stress-induced protein that, when overexpressed, promotes formation of intracellular vacuoles followed by cell death. May be involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis.VMP1 is a transmembrane protein that co-localizes with LC3, a marker of the autophagosomes. It interacts with beclin-1, a mammalian autophagy initiator, through the VMP1-Atg domain, which is essential for autophagosome formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ectopic P-granules autophagy protein 3 homolog; Transmembrane protein 49; transport and golgi organization 5 homolog; unnamed protein product; Vacuole membrane protein 1; VMP1
Gene Aliases: EPG3; HSPC292; TANGO5; TDC1; TMEM49; VMP1
UniProt ID: (Human) Q96GC9
Entrez Gene ID: (Human) 81671
autophagosome assembly
lipid transport
autophagy
Golgi organization
cell adhesion
embryo implantation
autophagosome membrane docking
phospholipid scrambling
cell junction assembly
lipoprotein transport
cell-cell adhesion
organelle localization by membrane tethering
positive regulation of calcium-transporting ATPase activity
mitochondrion-ER tethering
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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