Proteintech

VPS16 Polyclonal Antibody

VPS16 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

VPS16 Antibody (17776-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using (Product # 17776-1-AP) (VPS16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VPS16 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17776-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 8 publications 8 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

VPS16 Fusion Protein Ag12202 (177-525 aa encoded by BC073959)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KVQQKDVSD EDVARAINQK LGDTPGVSYS DIAARAYGCG RTELAIKLLE YEPRSGEQVP LLLKMKRSKL ALSKAIESGD TDLVFTVLLH LKNELNRGDF FMTLRNQPMA LSLYRQFCKH QELETLKDLY NQDDNHQELG SFHIRASYAA EERIEGRVAA LQTAADAFYK AKNEFAAKAT EDQMRLLRLQ RRLEDELGGQ FLDLSLHDTV TTLILGGHNK RAEQLARDFR IPDKRLWWLK LTALADLEDW EELEKFSKSK KSPIGYLPFV EICMKQHNKY EAKKYASRVG PEQKVKALLL VGDVAQAADV AIEHRNEAEL SLVLSHCTGA TDGATADKIQ RARAQAQKK (177-525 aa encoded by BC073959)

Target Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes.

Autophagy

Zheng W,Chen Q,Liu H,Zeng L,Zhou Y,Liu X,Bai Y,Zhang J,Pan Y,Shao C

Wed Mar 01 00:00:00 UTC 2023

Loss of Sirt1 promotes exosome secretion from podocytes by inhibiting lysosomal acidification in diabetic nephropathy.

Molecular and cellular endocrinology

Ding L,Li ZL,Zhou Y,Liu NC,Liu SS,Zhang XJ,Liu CC,Zhang DJ,Wang GH,Ma RX

Thu Jun 01 00:00:00 UTC 2023

AP2S1 regulates APP degradation through late endosome-lysosome fusion in cells and APP/PS1 mice.

Traffic (Copenhagen, Denmark)

Wen QX,Luo B,Xie XY,Zhou GF,Chen J,Song L,Liu Y,Xie SQ,Chen L,Li KY,Xiang XJ,Chen GJ

Sun Jan 01 00:00:00 UTC 2023

Contribution of Hepatic Steatosis-Intensified Extracellular Vesicle Release to Aggravated Inflammatory Endothelial Injury in Liver-Specific Asah1 Gene Knockout Mice.

The American journal of pathology

Yuan X,Bhat OM,Zou Y,Zhang Y,Li PL

Sat Apr 01 00:00:00 UTC 2023

Overexpression of VPS16 correlates with tumor progression and chemoresistance in colorectal cancer.

Biochemical and biophysical research communications

Zhang B,Ma Y,Niu H,Liu Z

Sat Jun 04 00:00:00 UTC 2022

CUR5g, a novel autophagy inhibitor, exhibits potent synergistic anticancer effects with cisplatin against non-small-cell lung cancer.

Cell death discovery

Chen J,Shen Y,Wu B,Yang P,Sun G,Liu X,Qiang P,Gao Y,Sha F,Li Z,Zhang L

Mon Oct 31 00:00:00 UTC 2022

Abnormal podocyte TRPML1 channel activity and exosome release in mice with podocyte-specific Asah1 gene deletion.

Biochimica et biophysica acta. Molecular and cell biology of lipids

Li G,Huang D,Bhat OM,Poklis JL,Zhang A,Zou Y,Kidd J,Gehr TWB,Li PL

Mon Feb 01 00:00:00 UTC 2021

Regulation of TRPML1 channel activity and inflammatory exosome release by endogenously produced reactive oxygen species in mouse podocytes.

Redox biology

Li G,Huang D,Li N,Ritter JK,Li PL

Thu Jul 01 00:00:00 UTC 2021

Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.

Autophagy

Shen Q,Shi Y,Liu J,Su H,Huang J,Zhang Y,Peng C,Zhou T,Sun Q,Wan W,Liu W

Sat May 01 00:00:00 UTC 2021

Contribution of podocyte inflammatory exosome release to glomerular inflammation and sclerosis during hyperhomocysteinemia.

Biochimica et biophysica acta. Molecular basis of disease

Huang D,Li G,Zhang Q,Bhat OM,Zou Y,Ritter JK,Li PL

Thu Jul 01 00:00:00 UTC 2021

Label-Free Quantitative Proteomic Profiling of LAD2 Mast Cell Releasates Reveals the Mechanism of Tween-80-Induced Anaphylactoid Reaction.

Proteomics. Clinical applications

Mi YN,Yan PP,Di J,Cao L,Xiao X,Liu DZ,Cao YX

Wed Jul 01 00:00:00 UTC 2020

Control of lysosomal TRPML1 channel activity and exosome release by acid ceramidase in mouse podocytes.

American journal of physiology. Cell physiology

Li G,Huang D,Hong J,Bhat OM,Yuan X,Li PL

Sun Sep 01 00:00:00 UTC 2019

Lysosomal regulation of extracellular vesicle excretion during d-ribose-induced NLRP3 inflammasome activation in podocytes.

Biochimica et biophysica acta. Molecular cell research

Hong J,Bhat OM,Li G,Dempsey SK,Zhang Q,Ritter JK,Li W,Li PL

Wed May 01 00:00:00 UTC 2019

Autophagosome maturation mediated by Rab7 contributes to neuroprotection of hypoxic preconditioning against global cerebral ischemia in rats.

Cell death & disease

Zhan L,Chen S,Li K,Liang D,Zhu X,Liu L,Lu Z,Sun W,Xu E

Thu Jul 20 00:00:00 UTC 2017

Autophagosome maturation mediated by Rab7 contributes to neuroprotection of hypoxic preconditioning against global cerebral ischemia in rats.

Cell death & disease

Zhan L,Chen S,Li K,Liang D,Zhu X,Liu L,Lu Z,Sun W,Xu E

Thu Jul 20 00:00:00 UTC 2017

Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.

The Journal of biological chemistry

van der Kant R,Jonker CT,Wijdeven RH,Bakker J,Janssen L,Klumperman J,Neefjes J

Fri Dec 18 00:00:00 UTC 2015

Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.

The Journal of biological chemistry

van der Kant R,Jonker CT,Wijdeven RH,Bakker J,Janssen L,Klumperman J,Neefjes J

Fri Dec 18 00:00:00 UTC 2015

Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.

The Journal of biological chemistry

van der Kant R,Jonker CT,Wijdeven RH,Bakker J,Janssen L,Klumperman J,Neefjes J

Fri Dec 18 00:00:00 UTC 2015

Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.

The Journal of biological chemistry

van der Kant R,Jonker CT,Wijdeven RH,Bakker J,Janssen L,Klumperman J,Neefjes J

Fri Dec 18 00:00:00 UTC 2015

Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.

Current biology : CB

Ulbricht A,Eppler FJ,Tapia VE,van der Ven PF,Hampe N,Hersch N,Vakeel P,Stadel D,Haas A,Saftig P,Behrends C,Fürst DO,Volkmer R,Hoffmann B,Kolanus W,Höhfeld J

Mon Mar 04 00:00:00 UTC 2013

Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.

Current biology : CB

Ulbricht A,Eppler FJ,Tapia VE,van der Ven PF,Hampe N,Hersch N,Vakeel P,Stadel D,Haas A,Saftig P,Behrends C,Fürst DO,Volkmer R,Hoffmann B,Kolanus W,Höhfeld J

Mon Mar 04 00:00:00 UTC 2013

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Journal of cell science

van der Kant R,Fish A,Janssen L,Janssen H,Krom S,Ho N,Brummelkamp T,Carette J,Rocha N,Neefjes J

Thu Aug 01 00:00:00 UTC 2013

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Journal of cell science

van der Kant R,Fish A,Janssen L,Janssen H,Krom S,Ho N,Brummelkamp T,Carette J,Rocha N,Neefjes J

Thu Aug 01 00:00:00 UTC 2013

Distinct autophagosomal-lysosomal fusion mechanism revealed by thapsigargin-induced autophagy arrest.

Molecular cell

Ganley IG,Wong PM,Gammoh N,Jiang X

Fri Jun 24 00:00:00 UTC 2011

Distinct autophagosomal-lysosomal fusion mechanism revealed by thapsigargin-induced autophagy arrest.

Molecular cell

Ganley IG,Wong PM,Gammoh N,Jiang X

Fri Jun 24 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: hVPS16; mVPS16; RP11-12M19.2; vacuolar protein sorting 16 homolog; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog; VPS16 CORVET/HOPS core subunit

View more View less

Gene Aliases: 1810074M16Rik; hVPS16; VPS16

View more View less

UniProt ID: (Human) Q9H269

View more View less

Entrez Gene ID: (Human) 64601, (Mouse) 80743, (Rat) 296159

View more View less

Function(s)

actin binding protein binding actin filament binding membrane traffic protein

Process(es)

intracellular protein transport endosome to lysosome transport regulation of vacuole fusion, non-autophagic regulation of SNARE complex assembly autophagosome maturation transport vacuole organization protein transport endosomal transport viral entry into host cell

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

VPS16 Polyclonal Antibody

VPS16 Antibody (17776-1-AP) in IHC (P)

Product Details

17776-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!