Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
VPS16 Antibody (PA5-90792) in WB
Western blot analysis of VPS16 in extracts of various cell lines. Samples were incubated with VPS16 Polyclonal antibody (Product # PA5-90792) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-90792
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 540-839 of human VPS16 (NP_0720972)
Conjugate
Form
Liquid
Concentration
1.52 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806239
Product Specific Information
Immunogen sequence: EYEPRSGEQV PLLLKMKRSK LALSKAIESG DTDLVFTVLL HLKNELNRGD FFMTLRNQPM ALSLYRQFCK HQELETLKDL YNQDDNHQEL GSFHIRASYA AEERIEGRVA ALQTAADAFY KAKNEFAAKA TEDQMRLLRL QRRLEDELGG QFLDLSLHDT VTTLILGGHN KRAEQLARDF RIPDKRLWWL KLTALADLED WEELEKFSKS KKSPIGYLPF VEICMKQHNK YEAKKYASRV GPEQKVKALL LVGDVAQAAD VAIEHRNEAE LSLVLSHCTG ATDGATADKI QRARAQAQKK; Positive Samples: A-549, Mouse brain, Mouse lung, Rat pancreas; Cellular Location: Cytoplasmic side, Cytoplasmic vesicle, Early endosome, Late endosome membrane, Lysosome membrane, Peripheral membrane protein, autophagosome, clathrin-coated vesicle
Target Information
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: hVPS16; mVPS16; RP11-12M19.2; vacuolar protein sorting 16 homolog; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog; VPS16 CORVET/HOPS core subunit
Gene Aliases: 1810074M16Rik; hVPS16; VPS16
UniProt ID: (Human) Q9H269
Entrez Gene ID: (Human) 64601, (Mouse) 80743, (Rat) 296159
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support