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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 4 and 221 of Human VPS28|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30553 targets VPS28 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-30553 immunogen is recombinant fragment corresponding to a region within amino acids 4 and 221 of Human VPS28.
This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ESCRT-I complex subunit VPS28; vacuolar protein sorting 28 homolog; vacuolar protein sorting 28-like protein; vacuolar protein sorting-associated protein 28 homolog; yeast class E protein Vps28p homolog