Immunohistochemistry analysis of VPS34 showing staining in the cytoplasm of paraffin-embedded mouse skeletal muscle tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temper... View More

VPS34 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

VPS34 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

VPS34 Antibody in Immunohistochemistry (IHC)

VPS34 Antibody in Western Blot, Immunoprecipitation (WB, IP)

VPS34 Antibody in Immunoprecipitation (IP)

Product Image Gallery

VPS34 Antibody — Fig. 6. Vps34 participates in NS4B- and HCV-induced autophagy. (A and B) GFP-LC3 cells were transfected with HA-NS4B (A) or infected with HCV JC1 virus (B). After 5 h, cells were treated with 3-MA at various concentrations for an additional 40 h of incubation and then fixed for indirect immunofluorescence staining with anti-HA or anticore antibody. GFP-LC3 (green) and HA-tagged NS4B or HCV core (red) are shown. (C and D) Huh7.5 cells were transduced with lentiviruses expressing shRNA against Vps34 (two different clones denoted as shVps34#1 [lanes 2 and 5] and shVps34#2 [lanes 3 and 6]) or shLacZ (as control [lanes 1 and 4]) and then treated with puromycin for selection of shRNA-carrying cells. At 4 days posttransduction, 1 x 10 5 of the transduced cells were reseeded into a 12-well plate for further experiments. The knockdown cells were transfected with 0.5 mug NS4B for 40 h (C) or infected with HCV JC1 virus at an MOI of 0.1 for 72 h (D). The cells were harvested, and the lysates were used for Western blot analysis with the indicated antibodies. The relative densitometric LC3-II/actin ratios are shown below the blots. Mock vector-transfected and noninfected cells were used as a control. {KD}

Product Details

38-2100

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 23 publications 23 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-dependent

View 6 publications 6 publications

Affinity Purification (AP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

C. elegans, Hamster, Human, Mouse, Non-human primate, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the mid region of the human VPS34 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533360

Target Information

PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: catalytic subunit type 3 Antibody; Class III PI 3-kinase; hVps34; hVps34 Antibody; OTTMUSP00000023341; OTTMUSP00000023350; phosphatidylinositol 3-kinase; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; phosphatidylinositol 3-kinase, catalytic subunit type 3; Phosphoinositide-3-kinase class 3; phosphoinositide-3-kinase, class 3; PI3-kinase type 3; PI3K type 3; pi3kc3; PIK3C3 Antibody; PtdIns-3-kinase type 3

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Gene Aliases: 5330434F23Rik; hVps34; PIK3C3; VPS34

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UniProt ID: (Human) Q8NEB9, (Mouse) Q6PF93

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Entrez Gene ID: (Human) 5289, (Mouse) 225326

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Function(s)

protein kinase activity protein binding ATP binding 1-phosphatidylinositol-3-kinase activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor phosphatidylinositol 3-kinase activity kinase

Process(es)

autophagosome assembly cytokinesis protein phosphorylation phosphatidylinositol biosynthetic process endosome organization macroautophagy protein processing regulation of cytokinesis toll-like receptor 9 signaling pathway phosphatidylinositol-3-phosphate biosynthetic process cellular response to glucose starvation response to leucine early endosome to late endosome transport phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling regulation of protein secretion endocytosis autophagy cell cycle endosomal transport phosphorylation pexophagy cell division

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VPS34 Polyclonal Antibody

VPS34 Antibody (38-2100) in IHC (P)

Product Details

38-2100

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Affinity Purification (AP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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