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Invitrogen
VPS34 Polyclonal Antibody
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FIGURE: 1 / 9
VPS34 Antibody (PA5-85581) in ICC/IF
Immunofluorescence analysis of Phosphatidylinositol 3-kinase catalytic subunit type 3 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with VPS34 Polyclonal Antibody (Product # PA5-85581) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody,... View More
Product Details
PA5-85581
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human VPS34. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (86%), Xenopus laevis (92%), Dog (99%), Pig (98%), Bovine (99%).
Positive Control: 293T, A431, HeLa, HepG2, U87-MG, SK-N-SH, IMR32, SK-N-AS, unboiled hVps34-transfected 293T cells.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: catalytic subunit type 3 Antibody; Class III PI 3-kinase; hVps34; hVps34 Antibody; OTTMUSP00000023341; OTTMUSP00000023350; phosphatidylinositol 3-kinase; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; phosphoinositide-3-kinase, class 3; PI3-kinase type 3; PI3K type 3; pi3kc3; PIK3C3 Antibody; PtdIns-3-kinase type 3; unnamed protein product; vacuolar protein sorting 34 homolog
Gene Aliases: hVps34; PIK3C3; VPS34
UniProt ID: (Human) Q8NEB9
Entrez Gene ID: (Human) 5289
autophagosome assembly
pexophagy
protein targeting to lysosome
lipid metabolic process
endocytosis
autophagy
endosome organization
cellular response to starvation
regulation of autophagy
macroautophagy
regulation of macroautophagy
protein processing
regulation of cytokinesis
protein localization to pre-autophagosomal structure
phosphatidylinositol-3-phosphate biosynthetic process
cellular response to glucose starvation
response to leucine
protein kinase B signaling
positive regulation by host of viral genome replication
early endosome to late endosome transport
positive regulation of natural killer cell mediated cytotoxicity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
synaptic vesicle endocytosis
cell division
interferon-gamma-mediated signaling pathway
interleukin-6-mediated signaling pathway
autophagosome maturation
positive regulation of protein lipidation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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