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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 70 and 268 of Human VPS36|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30611 targets VPS36 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-30611 immunogen is recombinant protein fragment corresponding to a region within amino acids 70 and 268 of Human VPS36.
VPS36, VPS22 (SNF8; MIM 610904), and VPS25 (MIM 610907) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS36, VPS22, and VPS25 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
45 kDa; C13orf9; EAP45; ELL-associated protein; ELL-associated protein of 45 kDa; ESCRT-II complex subunit VPS36; vacuolar protein-sorting-associated protein 36
C13orf9; CGI-145; EAP45; VPS36