Invitrogen

VPS4A Polyclonal Antibody

View all (8) VPS4A antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (5)

FIGURE: 1 / 5

VPS4A Antibody (PA5-110573) in WB

Western blot analysis of VPS4A in extracts of various cell lines. Samples were incubated with VPS4A Polyclonal antibody (Product # PA5-110573) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

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VPS4A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of VPS4A in NIH/3T3 cells. Samples were incubated with VPS4A Polyclonal antibody (Product # PA5-110573) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

VPS4A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of VPS4A in paraffin-embedded human colon carcinoma. Samples were incubated with VPS4A Polyclonal antibody (Product # PA5-110573) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Product Details

PA5-110573

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human VPS4A (NP_037377.1).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1.24 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2855984

Product Specific Information

Sequence of this protein is as follows: MTTSTLQKAI DLVTKATEED KAKNYEEALR LYQHAVEYFL HAIKYEAHSD KAKESIRAKC VQYLDRAEKL KDYLRSKEKH GKKPVKENQS EGKGSDSDSE GDNPEKKKLQ EQLMGAVVME

Target Information

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: hVPS4; Protein SKD2; SKD1-homolog; vacuolar protein sorting 4a; vacuolar protein sorting factor 4A; vacuolar protein sorting protein 4a; Vacuolar protein sorting-associated protein 4A; vacuolar sorting protein 4; VPS4-1; VPS4A

Gene Aliases: 4930589C15Rik; AI325971; AW553189; SKD1; SKD1A; SKD2; VPS4; VPS4-1; vps4-A; VPS4A

UniProt ID: (Human) Q9UN37, (Rat) Q793F9, (Mouse) Q8VEJ9

Entrez Gene ID: (Human) 27183, (Rat) 246772, (Mouse) 116733

Molecular Function: non-motor microtubule binding protein