Search
Bethyl Laboratories
VPS4B Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
VPS4B Antibody (A302-078A) in WB
Detection of human VPS4B by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-VPS4B antibody (Product # A302-078A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. VPS4B was also immunoprecipitated by rabbit anti-VPS4B antibody A302-077A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 minute (A) and 10 seconds (B)... View More
Product Details
A302-078A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 350 and 400 of Human vacuolar protein sorting 4 homolog B.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: cell migration-inducing 1; Cell migration-inducing gene 1 protein; MIG1; Protein SKD1; Suppressor of K(+) transport growth defect 1; suppressor of K+ transport defect 1; unnamed protein product; vacuolar protein sorting 4B; Vacuolar protein sorting-associated protein 4B
Gene Aliases: MIG1; SKD1; SKD1B; VPS4-2; VPS42; VPS4B
UniProt ID: (Human) O75351
Entrez Gene ID: (Human) 9525
angiogenesis
plasma membrane repair
potassium ion transport
autophagy
nucleus organization
endosome organization
vacuole organization
vacuolar transport
lysosomal transport
mitotic metaphase plate congression
central nervous system development
regulation of G2/M transition of mitotic cell cycle
regulation of centrosome duplication
positive regulation of G2/M transition of mitotic cell cycle
protein transport
endosomal transport
macroautophagy
cholesterol transport
nuclear envelope reassembly
endosome to lysosome transport via multivesicular body sorting pathway
response to lipid
multivesicular body assembly
viral budding via host ESCRT complex
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
viral budding from plasma membrane
protein depolymerization
cell division
canonical Wnt signaling pathway
establishment of blood-brain barrier
late endosomal microautophagy
late endosome to lysosome transport via multivesicular body sorting pathway
midbody abscission
multivesicular body sorting pathway
membrane fission
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
autophagosome maturation
regulation of mitotic spindle assembly
negative regulation of exosomal secretion
positive regulation of exosomal secretion
positive regulation of centriole elongation
ESCRT III complex disassembly
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support