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Invitrogen
VPS54 Polyclonal Antibody
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FIGURE: 1 / 1
VPS54 Antibody (PA5-89086) in WB
Western blot analysis of VPS54 in extracts of various cell lines. Samples were incubated with VPS54 Polyclonal antibody (Product # PA5-89086) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-89086
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 420-720 of human VPS54 (NP_0010057391)
Conjugate
Form
Liquid
Concentration
1.95 mg/mL
Amount
195 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: VVKLADQMRM LNFPQWFDLL KDIFSKFTIF LQRVKATLNI IHSVVLSVLD KNQRTRELEE ISQQKNAAKD NSLDTEVAYL IHEGMFISDA FGEGELTPIA VDTTSQRNAS PNSEPCSSDS VSEPECTTDS SSSKEHTSSS AIPGGVDIMV SEDMKLTDSE LGKLANNIQE LLYSASDICH DRAVKFLMSR AKDGFLEKLN SMEFITLSRL METFILDTEQ ICGRKSTSLL GALQSQAIKF VNRFHEERKT KLSLLLDNER WKQADVPAEF QDLVDSLSDG KIALPEKKSG ATEERKPAEV L; Positive Samples: 293T, Mouse kidney, Mouse lung; Cellular Location: Golgi apparatus, trans-Golgi network
Target Information
This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hepatocellular carcinoma protein 8; hVps54L; protein phosphatase 1, regulatory subunit 164; Tumor antigen HOM-HCC-8; Tumor antigen SLP-8p; Tumor antigen SLP-8p homolog; unnamed protein product; vacuolar protein sorting 54 homolog; Vacuolar protein sorting-associated protein 54; VPS54, GARP complex subunit
Gene Aliases: HCC8; hVps54L; mSLP8; PPP1R164; SLP-8p; VPS54; VPS54L; WR
UniProt ID: (Human) Q9P1Q0, (Mouse) Q5SPW0
Entrez Gene ID: (Human) 51542, (Mouse) 245944
in utero embryonic development
apoptotic DNA fragmentation
protein targeting to lysosome
protein targeting to vacuole
cellular calcium ion homeostasis
Golgi to vacuole transport
striated muscle contraction
mitochondrion organization
vacuole organization
lysosomal transport
neuromuscular synaptic transmission
skeletal muscle tissue development
protein localization
gene expression
ubiquitin homeostasis
microglia differentiation
protein transport
respiratory electron transport chain
sphingolipid catabolic process
protein localization to cell surface
limb morphogenesis
post-embryonic forelimb morphogenesis
synaptic transmission, glutamatergic
regulation of growth
retrograde transport, endosome to Golgi
protein targeting to ER
response to antibiotic
spermatid differentiation
skeletal muscle tissue growth
astrocyte differentiation
neuron projection morphogenesis
homeostasis of number of cells
homeostasis of number of cells within a tissue
musculoskeletal movement
neuron apoptotic process
response to calcium ion
synaptic transmission, GABAergic
L-glutamate import
neurofilament cytoskeleton organization
limb development
motor behavior
thrombin receptor signaling pathway
cellular response to progesterone stimulus
vesicle-mediated cholesterol transport
motor neuron apoptotic process
neural tissue regeneration
neuroinflammatory response
negative regulation of motor neuron apoptotic process
transport
growth
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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