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Invitrogen
VRK1 Polyclonal Antibody
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FIGURE: 1 / 5
VRK1 Antibody (PA5-96785) in IHC (P)
Immunohistochemistry analysis of VRK1 in paraffin-embedded mouse lung. Samples were incubated with VRK1 Polyclonal antibody (Product # PA5-96785) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-96785
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
4.74 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: GHLPWEDNLK DPKYVRDSKI RYRENIASLM DKCFPEKNKP GEIAKYMETV KLLDYTEKPL YENLRDILLQ GLKAIGSKDD GKLDLSVVEN GGLKAKTITK KRKKEIEESK EPGVEDTEWS NTQTEEAIQT RSRTRKRVQK; Positive Samples: MCF-7, Jurkat, A-549, HeLa; Cellular Location: Cytoplasm, Nucleus, cytoskeleton, spindle
Target Information
Human vaccinia-related kinase 1 (VRK1) is a new kinase related to a poxvirus kinase, and more distantly to the casein kinase 1 family. VRK1 shows strong autophosphorylating activity in several Ser and Thr residues. VRK has also been found to be an upstream regulator of p53 that belongs to a new signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC117401; MGC138280; Serine/threonine-protein kinase 51PK; Serine/threonine-protein kinase VRK1; vaccinia virus B1R-related kinase 1; Vaccinia-related kinase 1
Gene Aliases: 51PK; PCH1; PCH1A; VRK1
UniProt ID: (Human) Q99986, (Mouse) Q80X41
Entrez Gene ID: (Human) 7443, (Mouse) 22367, (Rat) 362779
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
protein kinase binding
nucleosomal histone binding
histone kinase activity (H3-S10 specific)
histone kinase activity (H3-T3 specific)
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein phosphorylation
mitotic nuclear division
mitotic nuclear envelope disassembly
mitotic nuclear envelope reassembly
regulation of cell shape
histone H3-S10 phosphorylation
protein autophosphorylation
cell division
histone H3-T3 phosphorylation
Golgi disassembly
endocytosis
cell cycle
Wnt signaling pathway
phosphorylation
peptidyl-serine phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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