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Invitrogen
Villin Polyclonal Antibody
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FIGURE: 1 / 3
Villin Antibody (PA5-17290) in WB
Western blot was performed using Anti-Villin Polyclonal Antibody (Product # PA5-17290) and a 92kDa band corresponding to Villin was observed across cell lines and tissue tested. Whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), COLO 205 (Lane 2), Jurkat (Lane 3) and PANC-1 (Lane 4) as seen in Fig (a).Tissue extracts of Mouse Liver (Lane 1), Mouse Colon (Lane 2) and Mouse Jejunum (Lane 3) as seen in Fig (b)were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a... View More
Product Details
PA5-17290
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the carboxy terminus of human villin-1
Conjugate
Form
Liquid
Concentration
30 µg/mL
Purification
Protein A
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: OTTHUMP00000207159; Villin-1
Gene Aliases: D2S1471; VIL; VIL1
UniProt ID: (Human) Q53S11, (Mouse) Q62468
Entrez Gene ID: (Human) 7429, (Mouse) 22349
calcium ion binding
protein binding
phosphatidylinositol-4,5-bisphosphate binding
lysophosphatidic acid binding
identical protein binding
protein homodimerization activity
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
actin filament binding
actin binding
metal ion binding
non-motor actin binding protein
intestinal D-glucose absorption
protein complex assembly
apoptotic process
epidermal growth factor receptor signaling pathway
regulation of cell shape
response to bacterium
positive regulation of epithelial cell migration
actin filament polymerization
actin filament depolymerization
positive regulation of cell migration
positive regulation of actin filament depolymerization
epithelial cell differentiation
positive regulation of actin filament bundle assembly
regulation of microvillus length
cellular response to hepatocyte growth factor stimulus
positive regulation of multicellular organism growth
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
actin nucleation
actin filament severing
barbed-end actin filament capping
regulation of actin nucleation
actin filament capping
cytoplasmic actin-based contraction involved in cell motility
regulation of wound healing
cellular response to epidermal growth factor stimulus
positive regulation of establishment of protein localization to plasma membrane
terminal web assembly
regulation of lamellipodium morphogenesis
positive regulation of lamellipodium morphogenesis
cytoskeleton organization
microvillus assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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