Invitrogen

Villin Polyclonal Antibody

Product Image Gallery

Villin Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Villin in methanol-fixed Hep3B cells using a Villin polyclonal antibody (Product # PA5-29078) at a 1:500 dilution.

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Villin in paraffin-embedded NCI-N87 xenograft using a Villin polyclonal antibody (Product # PA5-29078) at a dilution of 1:500.

Villin Antibody in Western Blot (WB) — Western blot analysis of Villin using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Villin polyclonal antibody (Product # PA5-29078) at a dilution of 1:10,000.

Villin Antibody — Published figure using Villin polyclonal antibody (Product # PA5-29078)

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:5000-1:20,000

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 7 and 286 of Human Villin

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2546554

Product Specific Information

Recommended positive controls: A431, H1299, HepG2, mouse kidney.

Predicted reactivity: Mouse (89%), Rat (89%), Bovine (90%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.