Invitrogen

Villin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

2 Published Figures

1 Reference

View all (20) Villin antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

Villin Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Villin in methanol-fixed Hep3B cells using a Villin polyclonal antibody (Product # PA5-29078) at a 1:500 dilution.

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Villin in paraffin-embedded NCI-N87 xenograft using a Villin polyclonal antibody (Product # PA5-29078) at a dilution of 1:500.

Villin Antibody in Western Blot (WB) — Western blot was performed using Anti-Villin Polyclonal Antibody (Product # PA5-29078) and a 92kDa band corresponding to Villin was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), COLO 205 (Lane 2), HT-29 (Lane 3), Jurkat (Lane 4) and PANC-1 (Lane 5) as seen in Fig (a).Tissue extracts of Mouse Liver (Lane 1), Mouse Colon (Lane 2) and Mouse Jejunum (Lane 3) as seen in Fig (b) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Villin Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of Villin was observed in High in Caco-2, COLO 205 and HT-29 as compared to low or negative in Jurkat and PANC-1 as seen in Fig (a),Similarly expression was observed high in Mouse Jejunum as compared to low or negative in Mouse Colon and Mouse Liver as seen in Fig (b) using Anti-Villin Polyclonal Antibody (Product # PA5-29078) in Western Blot. (Ref-http://dx.doi.org/10.1016/j.cbpa.2016.08.016, https://doi.org/10.1371/journal.pone.0084221). {RE}

Villin Antibody — Published figure using Villin polyclonal antibody (Product # PA5-29078)

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:20,000

Product Specifications

Species

Human, Mouse, Rat

Published species

Human

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 7 and 286 of Human Villin

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2546554

Product Specific Information

Recommended positive controls: A431, H1299, HepG2, mouse kidney.

Predicted reactivity: Mouse (89%), Rat (89%), Bovine (90%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.