Proteintech

Vimentin Polyclonal Antibody

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Antibody Testing Data (27)

FIGURE: 1 / 27

Vimentin Antibody (10366-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 10366-1-AP) Vimentin antibody at dilution of 1:5,000 incubated at room temperature for 1.5 hours.

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Vimentin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 10366-1-AP (Vimentin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue slide using (Product # 10366-1-AP) Vimentin antibody at dilution of 1:5,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Vimentin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed Human breast cancer tissue using Vimentin antibody (Product # 10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Vimentin Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug Vimentin antibody (10366-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

10366-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 1,040 publications 1,040 publications

Immunohistochemistry (IHC)

View 192 publications 192 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:4,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 252 publications 252 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 2 publications 2 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 10 publications 10 publications

Miscellaneous PubMed (Misc)

View 12 publications 12 publications

Not applicable (N/A)

View 22 publications 22 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Deer, Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Vimentin Fusion Protein Ag0489 (1-466 aa encoded by BC000163)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MSTRSVSSS SYRRMFGGPG TASRPSSSRS YVTTSTRTYS LGSALRPSTS RSLYASSPGG VYATRSSAVR LRSSVPGVRL LQDSVDFSLA DAINTEFKNT RTNEKVELQE LNDRFANYID KVRFLEQQNK ILLAELEQLK GQGKSRLGDL YEEEMRELRR QVDQLTNDKA RVEVERDNLA EDIMRLREKL QEEMLQREEA ENTLQSFRQD VDNASLARLD LERKVESLQE EIAFLKKLHE EEIQELQAQI QEQHVQIDVD VSKPDLTAAL RDVRQQYESV AAKNLQEAEE WYKSKFADLS EAANRNNDAL RQAKQESTEY RRQVQSLTCE VDALKGTNES LERQMREMEE NFAVEAANYQ DTIGRLQDEI QNMKEEMARH LREYQDLLNV KMALDIEIAT YRKLLEGEES RISLPLPNFS SLNLRETNLD SLPLVDTHSK RTLLIKTVET RDGQVINETS QHHDDLE (1-466 aa encoded by BC000163)

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.