|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 61 and 426 of Human Vimentin|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:50000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
PA5-27231 targets Vimentin in IF, IHC (P), and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-27231 immunogen is recombinant protein fragment corresponding to a region within amino acids 61 and 426 of Human Vimentin.
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Doxorubicin and cisplatin induce apoptosis in ovarian stromal cells obtained from cryopreserved human ovarian tissue.
PA5-27231 was used in immunocytochemistry to analyze the induction of apoptosis in ovarian stromal cells obtained from cryopreserved human ovarian tissue by cisplatin and doxorubicin
|Fabbri R,Macciocca M,Vicenti R,Paradisi R,Klinger FG,Pasquinelli G,Spisni E,Seracchioli R,Papi A||Future oncology (London, England) (12:1699)||2016|