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Invitrogen
Vinculin Polyclonal Antibody
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Product Details
PA5-82003
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human VCL. Recombinant protein control fragment (Product #RP-100519).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: KMIDERQQEL THQEHRVMLV NSMNTVKELL PVLISAMKIF VTTKNSKNQG IEEALKNRNF TVEKMSAEIN EIIRVLQLTS WDEDAWASKD TEAMKRALAS IDSKLNQAKG WLRDPSASPG DAGEQAIRQI LDEAGKVGEL CAGKERREI
Highest antigen sequence indentity to the following orthologs: Rat - 99%, Mouse - 99%.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis luminal protein 114; epididymis secretory sperm binding protein; meta-vinculin; Metavinculin; metavinculin {ECO:0000250|UniProtKB:P18206}; MV; RP11-178G16.3; unnamed protein product; vcl {ECO:0000250|UniProtKB:P18206}; vinc; Vinculin; vinculin {ECO:0000250|UniProtKB:P18206}
Gene Aliases: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; HEL114; MV; MVCL; VCL; VINC
UniProt ID: (Human) P18206, (Mouse) Q64727, (Rat) P85972
Entrez Gene ID: (Human) 7414, (Mouse) 22330, (Rat) 305679
morphogenesis of an epithelium
cell adhesion
cell-matrix adhesion
lamellipodium assembly
regulation of cell migration
negative regulation of cell migration
adherens junction assembly
protein localization to cell surface
maintenance of blood-brain barrier
apical junction assembly
axon extension
regulation of focal adhesion assembly
platelet aggregation
epithelial cell-cell adhesion
regulation of establishment of endothelial barrier
regulation of protein localization to cell-cell adherens junction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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