Invitrogen

Vitronectin Polyclonal Antibody

View all (23) Vitronectin antibodies

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Vitronectin Antibody in Western Blot (WB) — Western blot analysis was performed on Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 (Condition media) (Lane 2), Caco-2 (Lane 3) and Caco-2 (Condition media) (Lane 4). The blots were probed with Anti-Vitronectin Rabbit Polyclonal Antibody (Product # 720229, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 75 kDa band corresponding to Vitronectin was observed only in condition media tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Product Details

720229

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptides corresponding to human VTN (aa367-384, 107-120) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 30% glycerol, 0.1% BSA
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2662365

Product Specific Information

This antibody is predicted to react with Monkey, Dog, Mouse

Target Information

VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: complement S-protein; epibolin; S-protein; serum spreading factor; Serum-spreading factor; somatomedin B; Somatomedin-B; V75; Vitronectin; Vitronectin V10 subunit; Vitronectin V65 subunit; VN

Gene Aliases: V75; VN; VNT; VTN

UniProt ID: (Human) P04004

Entrez Gene ID: (Human) 7448

Function(s)

receptor-mediated endocytosis immune response cell adhesion cell-matrix adhesion positive regulation of cell-substrate adhesion negative regulation of endopeptidase activity positive regulation of smooth muscle cell migration negative regulation of blood coagulation extracellular matrix organization regulation of complement activation positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of protein binding cell adhesion mediated by integrin endodermal cell differentiation positive regulation of receptor-mediated endocytosis oligodendrocyte differentiation positive regulation of peptidyl-tyrosine phosphorylation smooth muscle cell-matrix adhesion positive regulation of wound healing

Process(es)

scavenger receptor activity integrin binding protein binding heparin binding polysaccharide binding extracellular matrix binding

Vitronectin Polyclonal Antibody

Product Details

720229

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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