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WAPL Antibody (PA5-143906) in ICC/IF
Immunocytochemistry analysis of WAPL in T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in WAPL Polyclonal antibody (Product # PA5-143906) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate fo... View More
Product Details
PA5-143906
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human WAPL/FOE recombinant protein (Position: Q916-C1190).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075120
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human 293T whole cell, human HepG2 whole cell, rat L6 whole cell, mouse C2C12 whole cell. IHC: mouse colon tissue, human lymphoma tissue, human stomach cancer tissue, human tonsil tissue. ICC/IF: T-47D cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Transcriptional repressor which may play a role in development of the central nervous system (CNS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DIF-2; Dioxin-inducible factor 2; friend of EBNA2; friend of EBNA2 (Epstein-Barr virus nuclear protein 2); Friend of EBNA2 protein; WAPL cohesin release factor; wings apart-like homolog (Drosophila); Wings apart-like protein homolog
Gene Aliases: A530089A20Rik; BC037674; DIF-2; FOE; KIAA0261; RGD1308960; WAPAL; WAPL
UniProt ID: (Human) Q7Z5K2, (Mouse) Q65Z40
Entrez Gene ID: (Human) 23063, (Rat) 290577, (Mouse) 218914
mitotic nuclear division
negative regulation of DNA replication
response to toxic substance
negative regulation of chromatin binding
negative regulation of sister chromatid cohesion
positive regulation of fibroblast proliferation
regulation of chromosome segregation
regulation of chromosome condensation
protein localization to chromatin
regulation of cohesin loading
sister chromatid cohesion
mitotic sister chromatid cohesion
viral process
meiotic chromosome segregation
cell division
cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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