|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||18 amino acid peptide near the amino terminus of human WAPL|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||20 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A recommended positive control is A-20 cell lysate.
At least four isoforms of WAPL are known to exist.
WAPL (wings apart-like), also known as WAPAL or FOE, is a 190 amino acid protein that contains one WAPL domain and may play an important role in cell growth. It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1). WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits. WAPL is a new regulator of the development and metastasis of cancerous tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.