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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • WARS Antibodies

          Invitrogen

          WARS Polyclonal Antibody

          View all (20) WARS antibodies

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          Cite WARS Polyclonal Antibody

          • Antibody Testing Data (2)
          WARS Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          WARS Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          WARS Antibody (PA5-112153) in WB

          Western Blot analysis of WARS using WARS Polyclonal Antibody (Product # PA5-112153) at a dilution of 1:500. Lane A: HepG2 whole cell lysate, Lane B: A431 whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG H&L (Dylight800) at 1:10,000 dilution. Developed using the Odyssey technique and performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 55 kDa. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          WARS Antibody in Western Blot (WB)
          WARS Antibody in Immunoprecipitation (IP)

          Product Details

          PA5-112153

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1:5,000-1:10,000
          -

          Immunoprecipitation (IP)

          1-4 µL/mg of lysate
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant Human WARS/TrpRS protein

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography, Protein A

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2866889

          Product Specific Information

          This product can be used in direct ELISA.

          Target Information

          Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: hWRS; IFI 53; IFP 53; IFP53; Interferon-induced protein 53; TrpRS; tryptophan tRNA ligase 1, cytoplasmic; Tryptophan--tRNA ligase, cytoplasmic; Tryptophanyl-tRNA synthetase; WARS protein

          View more View less

          Gene Aliases: GAMMA-2; IFI53; IFP53; WARS; WARS1; WRS

          View more View less

          UniProt ID: (Human) P23381

          View more View less

          Entrez Gene ID: (Human) 7453

          View more View less

          Function(s)
          tryptophan-tRNA ligase activity protein binding ATP binding aminoacyl-tRNA synthetase
          Process(es)
          angiogenesis translation tRNA aminoacylation for protein translation tryptophanyl-tRNA aminoacylation negative regulation of cell proliferation regulation of angiogenesis
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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