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Invitrogen
WARS Polyclonal Antibody
FIGURE: 1 / 13
WARS Antibody (PA5-29102) in ICC/IF
Immunofluorescence analysis of WARS was performed using 70% confluent log phase THP-1 cells treated with IFN-gamma (100 units/mL for 24h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with WARS Polyclonal Antibody (Product # PA5-29102) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody... View More
Product Details
PA5-29102
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 151 and 430 of Human Tryptophanyl tRNA synthetase
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2546578
Product Specific Information
Recommended positive controls: HepG2, mouse brain.
Predicted reactivity: Mouse (94%), Rat (93%), Zebrafish (86%), Xenopus laevis (89%), Rabbit (93%), Chimpanzee (100%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hWRS; IFI 53; IFP 53; IFP53; Interferon-induced protein 53; TrpRS; tryptophan tRNA ligase 1, cytoplasmic; Tryptophan--tRNA ligase, cytoplasmic; Tryptophanyl-tRNA synthetase; tryptophanyl-tRNA synthetase, cytoplasmic; WARS protein
Gene Aliases: GAMMA-2; IFI53; IFP53; TrpRS; WARS; WARS1; WRS
UniProt ID: (Human) P23381, (Mouse) P32921
Entrez Gene ID: (Human) 7453, (Rat) 314442, (Mouse) 22375
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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