Invitrogen

WBSCR22 Polyclonal Antibody

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WBSCR22 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of WBSCR22 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: WBSCR22 Polyclonal Antibody (Product # PA5-21698) diluted at 1:200. Red: FIS1, a mitochondria marker. Blue: Hoechst 33342 staining.

WBSCR22 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Western BlotSCR22 Polyclonal Antibody detects Western BlotSCR22 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Western BlotSCR22 Polyclonal Antibody (Product # PA5-21698) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

WBSCR22 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using Western BlotSCR22 (Product # PA5-21698) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

WBSCR22 Antibody in Western Blot (WB) — Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HCT116. 12% SDS PAGE. Western BlotSCR22 Polyclonal Antibody (Product # PA5-21698) diluted at 1:1,000.

WBSCR22 Antibody in Immunoprecipitation (IP) — Western BlotSCR22 Polyclonal Antibody immunoprecipitates Western BlotSCR22 protein in IP experiments. IP Sample: 1,000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of Western BlotSCR22 protein by 2.5 µg of Western BlotSCR22 Polyclonal Antibody (Product # PA5-21698) 12% SDS-PAGE The immunoprecipitated Western BlotSCR22 protein was detected by Western BlotSCR22 Polyclonal Antibody (Product # PA5-21698) diluted at 1:1,000.

Product Details

PA5-21698

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:500-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 1 and 263 of WBSCR22 (Uniprot ID#O43709) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.42 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 10% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11152715

Product Specific Information

Recommended positive controls: HeLa, HCT116, A375M.

Predicted reactivity: Rhesus Monkey (99%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bud site selection protein 23 homolog; Metastasis-related methyltransferase 1; Probable 18S rRNA (guanine-N(7))-methyltransferase; ribosome biogenesis methyltransferase WBSCR22; rRNA methyltransferase and ribosome maturation factor; Williams Beuren syndrome chromosome region 22; Williams-Beuren candidate region putative methyltransferase; Williams-Beuren syndrome chromosomal region 22 protein; Williams-Beuren syndrome chromosomal region 22 protein homolog

Gene Aliases: 1110003N24Rik; BUD23; HASJ4442; HUSSY-03; HUSSY-3; MERM1; PP3381; WBMT; WBSCR22

UniProt ID: (Human) O43709, (Mouse) Q9CY21

Entrez Gene ID: (Human) 114049, (Mouse) 66138

Molecular Function: methyltransferase transferase metabolite interconversion enzyme

transcription, DNA-templated regulation of transcription, DNA-templated biological_process covalent chromatin modification rRNA methylation rRNA processing metabolic process chromatin modification methylation ribosome biogenesis

Process(es)

methyltransferase activity poly(A) RNA binding rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity