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FIGURE: 1 / 3
WDYHV1 Antibody (PA5-78518) in ICC/IF
WDYHV1 Polyclonal Antibody detects WDYHV1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WDYHV1 protein stained by WDYHV1 Polyclonal Antibody (Product # PA5-78518) diluted at 1:200. Blue: Hoechst 33342 staining.
Product Details
PA5-78518
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human WDYHV1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.66 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 0.1M glycine, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736795
Product Specific Information
Predicted Reactivity: Rat (88%), Rhesus Monkey (97%), Bovine (83%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: N-terminal Gln amidase; nt(Q)-amidase; Protein N-terminal glutamine amidohydrolase; Protein NH2-terminal glutamine deamidase; WDYHV motif-containing protein 1
Gene Aliases: 2410187C16Rik; AU014961; AW550036; C8orf32; NTAQ1; WDYHV1
UniProt ID: (Human) Q96HA8, (Mouse) Q80WB5
Entrez Gene ID: (Human) 55093, (Mouse) 76773
protein binding
protein-N-terminal glutamine amidohydrolase activity
histone deacetylase activity
protein-N-terminal asparagine amidohydrolase activity
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
iprodione amidohydrolase activity
(3,5-dichlorophenylurea)acetate amidohydrolase activity
4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity
didemethylisoproturon amidohydrolase activity
N-isopropylacetanilide amidohydrolase activity
N-cyclohexylformamide amidohydrolase activity
isonicotinic acid hydrazide hydrolase activity
cis-aconitamide amidase activity
gamma-N-formylaminovinylacetate hydrolase activity
N2-acetyl-L-lysine deacetylase activity
O-succinylbenzoate synthase activity
indoleacetamide hydrolase activity
N-acetylcitrulline deacetylase activity
N-acetylgalactosamine-6-phosphate deacetylase activity
diacetylchitobiose deacetylase activity
chitooligosaccharide deacetylase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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