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Agrisera
WHIRLY1 Polyclonal Antibody
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FIGURE: 1 / 1
WHIRLY1 Antibody (AS16-3953) in IHC (PFA)
Material: Barley(flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White Samples: ultrathin sections on Au-Grids Immunogold labelling: 1. Blocking 30 min RT in PBS (p H 7,4) + 0,2% BSA + 0,2% fish gelatin (= blocking buffer) sterile filtrated 2. Incubation over night at 4°C anti-W 1 (goat) 1:200 in blocking buffer 3. Washing: 4 x 3 min PBS + 0,1% BSA (= washing buffer) 4. Incuba... View More
Product Details
AS16-3953
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from HvWHIRLY1 protein sequence.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Specific Species Reactivity: Hordeum vulgare
Target Information
WHIRLY1 encodes a plant Whirly-family nucleic-acid-binding protein that is predominantly associated with chloroplast nucleoids (including the transcriptionally active chromosome fraction) and can also be detected in the nucleus, consistent with a role in plastid-nucleus communication. It is synthesized as a nuclear-encoded precursor bearing an N-terminal plastid transit peptide and accumulates in developing chloroplasts where it associates with thylakoid-attached nucleoid DNA. Structurally, WHIRLY proteins form higher-order oligomers (commonly tetramers) and bind single-stranded DNA in a largely sequence-independent manner, while also exhibiting RNA-binding activity to subsets of plastid transcripts. Functionally, WHIRLY1 acts as a major architectural component of chloroplast nucleoids that promotes proper nucleoid organization and DNA compaction, influencing plastid DNA metabolism and coordinating chloroplast gene expression programs needed for chloroplast biogenesis (including effects on plastid ribosome accumulation and balanced assembly of plastid- and nucleus-encoded photosynthetic complexes). In the nucleus, WHIRLY1 can bind promoter regions of specific target genes to modulate transcriptional outputs linked to development and stress responses (e.g., regulation of senescence-associated pathways), supporting its proposed dual organelle-nucleus regulatory role.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATWHY1; p24; PTAC1; WHY1
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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