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Agrisera
WHIRLY2 Polyclonal Antibody
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FIGURE: 1 / 1
WHIRLY2 Antibody (AS16-3952) in IHC (PFA)
Material: Hordeum vulgare (flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White Samples: ultrathin sections on Au-Grids Immunogold labelling: 1. Blocking 30 min RT in PBS (p H 7,4) + 0,2% BSA + 0,2% fish gelatin (= blocking buffer) sterile filtrated 2. Incubation over night at 4°C anti-WHIRLY 2 (chicken antibodies) 1:100 in blocking buffer 3. Washing: 4 x 3 min PBS + 0,1% BSA... View More
Product Details
AS16-3952
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Chicken
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from HvWHIRLY2 protein sequence.
Conjugate
Form
Liquid
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 8
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunogen affinity purified IgY
Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Hordeum vulgare
Target Information
WHIRLY2 is a nucleus-encoded, plant-specific nucleic-acid-binding protein that localizes predominantly to mitochondria, where it is enriched in mitochondrial nucleoids and associates with mtDNA-containing complexes. It is synthesized with an N-terminal organelle-targeting presequence that is removed upon import, yielding a mature protein whose core is the conserved WHIRLY domain, an ssDNA-binding module typified by the KGKAAL motif; WHIRLY-family structural studies indicate this domain supports oligomerization into tetramers that can further assemble into higher-order particles on long ssDNA. Functionally, WHIRLY2 acts as an architectural nucleoid factor that binds ssDNA largely without strong sequence preference to promote mtDNA organization/compaction and to support mitochondrial genome stability; genetic disruption in Arabidopsis leads to disordered nucleoids and altered mitochondrial ultrastructure (including reduced cristae organization) accompanied by impaired mitochondrial performance, and loss-of-function also compromises mtDNA integrity/repair under stress (e.g., salt stress) with accumulation of aberrant recombination products and heightened stress sensitivity. Collectively, WHIRLY2 links nucleoid structure to mitochondrial morphology and bioenergetic competence, helping maintain mitochondrial genome maintenance and stress-responsive mitochondrial function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT1G71260; ATWHY2; F3I17.9; F3I17_9; WHIRLY 2; WHY2
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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