Western blot analysis of WIPI1 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human WIPI1-transfected 293T cells. Samples were loaded onto a 10% SDS-PAGE gel and probed with a WIPI1 polyclonal antibody (Product # PA5-34973) at a dilution of 1:10000.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide contains a sequence corresponding to a region within amino acids 356 and 446 of WIPI1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34973 targets WIPI1 in WB applications and shows reactivity with Human and Mouse samples.
The PA5-34973 immunogen is synthetic peptide contains a sequence corresponding to a region within amino acids 356 and 446 of WIPI1.
WIPI1 (WD repeat domain, phosphoinositide interacting-1), also known as WIPI1, ATG18 or WIPI49, is thought to play a role in autophagy and may regulate protein trafficking in certain recycling pathways. It contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. WIPI1 localizes to cytoplasmic vesicles, endosomes, clathrin-coated vesicles and the trans-Golgi network. It is ubiquitously expressed with highest expression in heart, testis, placenta, pancreas and skeletal muscle. WIPI1 is upregulated in a variety of tumors, suggesting a role in carcinogenesis.
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