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WISP2 Antibody (500-P212-100UG) in WB
Western Blot: To detect Human CTGFL/WISP-2 by Western Blot analysis WISP2 Polyclonal Antibody (Product # 500-P212-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTGFL/WISP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P212-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human CTGFL/WISP-2
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929837
Product Specific Information
AA Sequence of recombinant protein: MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CTGFL/WISP-2. Anti-Human CTGFL/WISP-2-specific antibody was purified by affinity chromatography employing an immobilized Human CTGFL/WISP-2 matrix.
Sandwich ELISA: To detect Human CTGFL/WISP-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human CTGFL/WISP-2 (500-P212Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CTGFL/WISP-2.
Western Blot: To detect Human CTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTGFL/WISP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Wisp2 a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. Wisp2 lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58; CTGF-L; WISP-2; WNT1-inducible-signaling pathway protein 2
Gene Aliases: CCN5; CT58; CTGF-L; CTGFL; UNQ228/PRO261; WISP2
UniProt ID: (Human) O76076
Entrez Gene ID: (Human) 8839
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