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          • Primary Antibodies ›
          • WNT5A Antibodies

          Invitrogen

          WNT5A Polyclonal Antibody

          1 Published Figure
          1 Reference
          View all (23) WNT5A antibodies

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          Cite WNT5A Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          • Published Figures (1)
          WNT5A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          WNT5A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          WNT5A Antibody (PA5-95181) in IHC (P)

          Immunohistochemistry (Paraffin) analysis of WNT5A in paraffin-embedded section of human lung cancer tissue using WNT5A Polyclonal Antibody (Product # PA5-95181). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 1 µg/mL dilution overnight a... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          WNT5A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          WNT5A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          WNT5A Antibody in Western Blot (WB)
          WNT5A Antibody in Immunohistochemistry (IHC)
          WNT5A Polyclonal Antibody

          Product Details

          PA5-95181

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          -

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          0.5-1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human Wnt5a recombinant protein (Position: D184-D303).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS with 5mg BSA

          Contains

          0.05mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2806986

          Product Specific Information

          Human Wnt5a shares 100% amino acid (aa) sequences identity with both mouse and rat Wnt5a.

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: human Hela whole cell, human PC-3 whole cell. IHC: human placenta tissue, human lung cancer tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          Wnt5A is a secreted protein found in the extracellular region that belongs to the Wnt family. It is a proto-oncogene that functions in modulating cell fate and behavior during vertebrate development and has an important role in Wnt signaling pathway. It is a glycoprotein that plays an important role in cell communication and development. Wnt5A acts as a ligand for members of the frizzled family of seven transmembrane receptors. It is suspected that Wnt5A is a developmental protein. Wnt5A is a signaling molecule which affects the development of discrete regions of tissues and is likely to signal over only few cell diameters.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: epididymis secretory sperm binding protein; hWNT5A; OTTHUMP00000212837; OTTHUMP00000212861; Protein Wnt-5a; unnamed protein product; wingless-related MMTV integration site 5A; wingless-type MMTV integration site 5A; wingless-type MMTV integration site family, member 5A; WNT-5A protein

          View more View less

          Gene Aliases: 8030457G12Rik; hWNT5A; Wnt-5a; WNT5A

          View more View less

          UniProt ID: (Human) P41221, (Mouse) P22725

          View more View less

          Entrez Gene ID: (Human) 7474, (Rat) 64566, (Mouse) 22418

          View more View less

          Function(s)
          receptor binding frizzled binding receptor tyrosine kinase-like orphan receptor binding cytokine activity protein binding phospholipid binding protein domain specific binding receptor agonist activity chemoattractant activity involved in axon guidance molecular_function transcription factor activity, sequence-specific DNA binding frizzled-2 binding transcription regulatory region DNA binding intercellular signal molecule
          Process(es)
          establishment of planar polarity somitogenesis positive regulation of cytokine production kidney development epithelial to mesenchymal transition neural tube closure positive regulation of endothelial cell proliferation heart looping morphogenesis of an epithelium positive regulation of mesenchymal cell proliferation lens development in camera-type eye positive regulation of cytokine production involved in immune response primary heart field specification secondary heart field specification ventricular septum development atrial septum development type B pancreatic cell development pericardium morphogenesis axis elongation optic cup formation involved in camera-type eye development inflammatory response phospholipase C-activating G-protein coupled receptor signaling pathway Wnt signaling pathway, calcium modulating pathway JNK cascade determination of left/right symmetry axonogenesis axon guidance hindgut morphogenesis midgut development protein localization cell proliferation fibroblast growth factor receptor signaling pathway male gonad development anterior/posterior axis specification, embryo anterior/posterior pattern specification mesenchymal cell proliferation positive regulation of endothelial cell migration positive regulation of gene expression epithelial cell migration macrophage derived foam cell differentiation positive regulation of T cell chemotaxis positive regulation of neuron projection development Wnt signaling pathway cell migration olfactory bulb interneuron development neural tube development neurogenesis cell differentiation neuron differentiation keratinocyte differentiation lung development embryonic limb morphogenesis BMP signaling pathway negative regulation of BMP signaling pathway midbrain development positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production regulation of protein localization positive regulation of Rac protein signal transduction limb morphogenesis non-canonical Wnt signaling pathway post-anal tail morphogenesis chemoattraction of serotonergic neuron axon chemorepulsion of dopaminergic neuron axon negative regulation of fibroblast growth factor receptor signaling pathway wound healing inner ear morphogenesis embryonic digit morphogenesis positive regulation of macrophage activation negative regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade cell fate commitment establishment of epithelial cell apical/basal polarity negative regulation of fat cell differentiation positive regulation of protein catabolic process positive regulation of angiogenesis positive regulation of ossification positive regulation of endocytosis positive regulation of meiotic nuclear division negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade development of primary male sexual characteristics negative regulation of melanin biosynthetic process positive regulation of fibroblast proliferation paraxial mesoderm formation digestive tract morphogenesis notochord morphogenesis embryonic skeletal system development genitalia development neuron projection morphogenesis negative regulation of axon extension involved in axon guidance axon extension involved in axon guidance hypophysis morphogenesis epithelial cell proliferation positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation regulation of inflammatory response positive regulation of inflammatory response regulation of synapse organization negative chemotaxis cartilage development positive regulation of anagen positive regulation of protein kinase B signaling negative regulation of synapse assembly convergent extension convergent extension involved in axis elongation convergent extension involved in organogenesis uterus development cervix development vagina development canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway urinary bladder development face development positive regulation of type I interferon-mediated signaling pathway lateral sprouting involved in mammary gland duct morphogenesis tube closure mesenchymal-epithelial cell signaling negative regulation of prostatic bud formation mammary gland branching involved in thelarche epithelial cell proliferation involved in mammary gland duct elongation positive regulation of response to cytokine stimulus regulation of branching involved in mammary gland duct morphogenesis mesodermal to mesenchymal transition involved in gastrulation positive regulation of macrophage cytokine production positive regulation of cartilage development somite development secondary palate development thymocyte apoptotic process positive regulation of thymocyte apoptotic process cellular response to molecule of bacterial origin cellular response to lipopolysaccharide cellular response to calcium ion cellular response to retinoic acid cellular response to interferon-gamma hematopoietic stem cell proliferation dopaminergic neuron differentiation cellular response to transforming growth factor beta stimulus stem cell proliferation negative regulation of mesenchymal cell proliferation primitive streak formation negative regulation of canonical Wnt signaling pathway cochlea morphogenesis melanocyte proliferation presynapse assembly postsynapse assembly postsynaptic modulation of chemical synaptic transmission regulation of postsynapse organization regulation of postsynaptic cytosolic calcium ion concentration meiotic nuclear division positive regulation of neuron projection arborization positive regulation of protein kinase C activity positive regulation of hematopoietic stem cell proliferation positive regulation of protein localization to synapse excitatory synapse assembly inhibitory synapse assembly negative regulation of cell proliferation in midbrain midbrain dopaminergic neuron differentiation positive regulation of cell-cell adhesion mediated by cadherin positive regulation of non-canonical Wnt signaling pathway positive regulation of stem cell proliferation positive regulation of apoptotic signaling pathway activation of MAPK activity ameboidal-type cell migration positive regulation of protein phosphorylation positive regulation of cytokine secretion involved in immune response protein phosphorylation activation of JUN kinase activity positive regulation of cell proliferation response to organic substance positive regulation of peptidyl-threonine phosphorylation response to organic cyclic compound positive regulation of cell-cell adhesion positive regulation of cGMP metabolic process prostate gland development activation of protein kinase B activity response to estradiol positive regulation of peptidyl-serine phosphorylation response to testosterone cellular protein localization non-canonical Wnt signaling pathway via JNK cascade positive regulation of JUN kinase activity estrous cycle positive regulation of chemokine biosynthetic process lung alveolus development positive regulation of interleukin-1 beta secretion positive regulation of NF-kappaB transcription factor activity response to glucocorticoid response to hyperoxia palate development planar cell polarity pathway involved in outflow tract morphogenesis planar cell polarity pathway involved in ventricular septum morphogenesis planar cell polarity pathway involved in cardiac right atrium morphogenesis planar cell polarity pathway involved in cardiac muscle tissue morphogenesis planar cell polarity pathway involved in pericardium morphogenesis positive regulation of protein kinase C signaling planar cell polarity pathway involved in neural tube closure positive regulation of interleukin-8 secretion signal transduction cell-cell signaling multicellular organism development embryo development organ morphogenesis
          It has to be done as per old AB suggested Products section.

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