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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • WNT5A/WNT5B Antibodies

          Proteintech

          WNT5A/B Polyclonal Antibody

          6 References

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          Cite WNT5A/B Polyclonal Antibody

          • Antibody Testing Data (2)
          WNT5A/B Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          WNT5A/B Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          WNT5A/B Antibody (29793-1-AP) in ICC/IF

          Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT5A antibody (29793-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          WNT5A/B Antibody in Immunocytochemistry (ICC/IF)
          WNT5A/B Antibody in Western Blot (WB)
          WNT5A/B Polyclonal Antibody

          Product Details

          29793-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 3 publications 3 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 3 publications 3 publications

          ELISA (ELISA)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          WNT5A/B fusion protein Ag31507
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.4 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice

          Product Specific Information

          Immunogen sequence: EGMDGCELMC CGRGYDQFKT VQTERCHCKF HWCCYVKCKK CTEIVDQFVC K

          Target Information

          The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Protein Wnt-5a; Protein Wnt-5b; wingless-type MMTV integration site family member 5A; wingless-type MMTV integration site family member 5B; wingless-type MMTV integration site family, member 5A; wingless-type MMTV integration site family, member 5B; WNT-5A protein; WNT-5B protein

          View more View less

          Gene Aliases: hWNT5A; WNT5A; WNT5B

          View more View less

          UniProt ID: (Human) P41221, (Human) Q9H1J7

          View more View less

          Entrez Gene ID: (Human) 7474, (Human) 81029

          View more View less

          Function(s)
          transcription factor activity, sequence-specific DNA binding frizzled binding frizzled-2 binding receptor tyrosine kinase-like orphan receptor binding cytokine activity protein binding protein domain specific binding transcription regulatory region DNA binding receptor agonist activity chemoattractant activity involved in axon guidance receptor binding intercellular signal molecule
          Process(es)
          activation of MAPK activity establishment of planar polarity somitogenesis epithelial to mesenchymal transition positive regulation of endothelial cell proliferation heart looping positive regulation of mesenchymal cell proliferation lens development in camera-type eye positive regulation of cytokine secretion involved in immune response type B pancreatic cell development planar cell polarity pathway involved in axis elongation optic cup formation involved in camera-type eye development Wnt signaling pathway, calcium modulating pathway activation of JUN kinase activity axon guidance synapse assembly hindgut morphogenesis midgut development male gonad development anterior/posterior axis specification, embryo response to organic substance positive regulation of endothelial cell migration positive regulation of peptidyl-threonine phosphorylation positive regulation of T cell chemotaxis positive regulation of neuron projection development Wnt signaling pathway olfactory bulb interneuron development neuron differentiation keratinocyte differentiation negative regulation of BMP signaling pathway positive regulation of cGMP metabolic process prostate gland development positive regulation of protein binding activation of protein kinase B activity response to estradiol positive regulation of interferon-gamma production positive regulation of interleukin-6 production positive regulation of peptidyl-serine phosphorylation response to testosterone cellular protein localization non-canonical Wnt signaling pathway post-anal tail morphogenesis chemoattraction of serotonergic neuron axon chemorepulsion of dopaminergic neuron axon non-canonical Wnt signaling pathway via JNK cascade negative regulation of fibroblast growth factor receptor signaling pathway wound healing embryonic digit morphogenesis positive regulation of macrophage activation negative regulation of apoptotic process estrous cycle positive regulation of chemokine biosynthetic process cell fate commitment establishment of epithelial cell apical/basal polarity negative regulation of fat cell differentiation positive regulation of protein catabolic process positive regulation of angiogenesis positive regulation of ossification positive regulation of endocytosis positive regulation of meiotic nuclear division negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of melanin biosynthetic process positive regulation of fibroblast proliferation lung alveolus development paraxial mesoderm formation notochord morphogenesis embryonic skeletal system development genitalia development negative regulation of axon extension involved in axon guidance hypophysis morphogenesis negative regulation of epithelial cell proliferation positive regulation of interleukin-1 beta secretion positive regulation of inflammatory response positive regulation of NF-kappaB transcription factor activity cartilage development response to glucocorticoid negative regulation of synapse assembly response to hyperoxia palate development convergent extension involved in axis elongation convergent extension involved in organogenesis uterus development cervix development vagina development canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway urinary bladder development face development positive regulation of type I interferon-mediated signaling pathway lateral sprouting involved in mammary gland duct morphogenesis mesenchymal-epithelial cell signaling negative regulation of prostatic bud formation mammary gland branching involved in thelarche epithelial cell proliferation involved in mammary gland duct elongation positive regulation of response to cytokine stimulus regulation of branching involved in mammary gland duct morphogenesis planar cell polarity pathway involved in gastrula mediolateral intercalation mesodermal to mesenchymal transition involved in gastrulation positive regulation of macrophage cytokine production positive regulation of cartilage development planar cell polarity pathway involved in outflow tract morphogenesis planar cell polarity pathway involved in ventricular septum morphogenesis planar cell polarity pathway involved in cardiac right atrium morphogenesis planar cell polarity pathway involved in cardiac muscle tissue morphogenesis planar cell polarity pathway involved in pericardium morphogenesis positive regulation of thymocyte apoptotic process cellular response to lipopolysaccharide cellular response to calcium ion cellular response to retinoic acid cellular response to interferon-gamma hematopoietic stem cell proliferation cellular response to transforming growth factor beta stimulus negative regulation of mesenchymal cell proliferation primitive streak formation positive regulation of protein kinase C signaling negative regulation of canonical Wnt signaling pathway cochlea morphogenesis planar cell polarity pathway involved in neural tube closure activation of GTPase activity melanocyte proliferation positive regulation of protein kinase C activity positive regulation of protein localization to synapse regulation of cellular protein localization excitatory synapse assembly inhibitory synapse assembly negative regulation of cell proliferation in midbrain Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation planar cell polarity pathway involved in midbrain dopaminergic neuron differentiation positive regulation of cell-cell adhesion mediated by cadherin positive regulation of non-canonical Wnt signaling pathway positive regulation of interleukin-8 secretion chondrocyte differentiation positive regulation of cell migration fat cell differentiation positive regulation of fat cell differentiation lens fiber cell development
          It has to be done as per old AB suggested Products section.
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