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WNT5B Antibody (PA5-116129) in ICC/IF
Immunocytochemistry analysis of WNT5B in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116129). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-116129
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human WNT5B(Accession Q9H1J7), corresponding to amino acid residues C161-K211.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900763
Product Specific Information
Antibody detects endogenous levels of total WNT5B.
Target Information
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein Wnt-5b; wingless-related MMTV integration site 5B; wingless-type MMTV integration site family member 5B; wingless-type MMTV integration site family, member 5B; WNT-5B protein
Gene Aliases: AW545702; Wnt-5b; WNT5B
UniProt ID: (Human) Q9H1J7, (Mouse) P22726
Entrez Gene ID: (Human) 81029, (Rat) 282582, (Mouse) 22419
chondrocyte differentiation
Wnt signaling pathway
neuron differentiation
positive regulation of cell migration
wound healing
cell fate commitment
fat cell differentiation
positive regulation of fat cell differentiation
lens fiber cell development
cellular response to retinoic acid
negative regulation of canonical Wnt signaling pathway
signal transduction
cell-cell signaling
multicellular organism development
organ morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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