Proteintech

WRAP53 Polyclonal Antibody

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WRAP53 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

WRAP53 Antibody in Western Blot (WB) — Human kidney tissue were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

WRAP53 Antibody in Immunoprecipitation (IP) — IP result of anti-WRAP53 (IP:14761-1-AP, 4ug; Detection:14761-1-AP 1:300) with HeLa cells lysate 2480ug.

Product Details

14761-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

WRAP53 Fusion Protein Ag6522 (166-548 aa encoded by BC002336)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: TQPENFLKG CKWAPDGSCI LTNSADNILR IYNLPPELYH EGEQVEYAEM VPVLRMVEGD TIYDYCWYSL MSSAQPDTSY VASSSRENPI HIWDAFTGEL RASFRAYNHL DELTAAHSLC FSPDGSQLFC GFNRTVRVFS TARPGRDCEV RATFAKKQGQ SGIISCIAFS PAQPLYACGS YGRSLGLYAW DDGSPLALLG GHQGGITHLC FHPDGNRFFS GARKDAELLC WDLRQSGYPL WSLGREVTTN QRIYFDLDPT GQFLVSGSTS GAVSVWDTDG PGNDGKPEPV LSFLPQKDCT NGVSLHPSLP LLATASGQRV FPEPTESGDE GEELGLPLLS TRHVHLECRL QLWWCGGAPD SSIPDDHQGE KGQGGTEGGV GELI (166-548 aa encoded by B C002336)

Target Information

TXNDC1 is a thioredoxin -related protein with disulfide reductase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TCAB1 prevents nucleolar accumulation of the telomerase RNA to facilitate telomerase assembly.

Cell reports

Klump BM,Perez GI,Patrick EM,Adams-Boone K,Cohen SB,Han L,Yu K,Schmidt JC

Thu Jun 01 00:00:00 EDT 2023

TCAB1 prevents nucleolar accumulation of the telomerase RNA to facilitate telomerase assembly.

Cell reports

Klump BM,Perez GI,Patrick EM,Adams-Boone K,Cohen SB,Han L,Yu K,Schmidt JC

Thu Jun 01 00:00:00 EDT 2023

Differential effects of WRAP53 transcript variants on non-small cell lung cancer cell behaviors.

PloS one

Zhu Y,Sun W,Jiang X,Bai R,Luo Y,Gao Y,Li S,Huang Z,Gong Y,Xie C

Fri Feb 03 00:00:00 EST 2023

RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.

Oncogenesis

Tan Q,Niu K,Zhu Y,Chen Z,Li Y,Li M,Wei D,Balajee AS,Fang H,Zhao Y

Fri Mar 05 00:00:00 EST 2021

Suppression of TCAB1 expression induced cellular senescence by lessening proteasomal degradation of p21 in cancer cells.

Cancer cell international

Niu J,Gao RQ,Cui MT,Zhang CG,Li ST,Cheng S,Ding W

Thu Jan 07 00:00:00 EST 2021

Observation of processive telomerase catalysis using high-resolution optical tweezers.

Nature chemical biology

Patrick EM,Slivka JD,Payne B,Comstock MJ,Schmidt JC

Wed Jul 01 00:00:00 EDT 2020

Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism.

Aging

Pan XH,Chen YH,Yang YK,Zhang XJ,Lin QK,Li ZA,Cai XM,Pang RQ,Zhu XQ,Ruan GP

Wed Jan 23 00:00:00 EST 2019

Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.

RNA biology

Coucoravas C,Dhanjal S,Henriksson S,Böhm S,Farnebo M

Sat Jun 03 00:00:00 EDT 2017

Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.

RNA biology

Coucoravas C,Dhanjal S,Henriksson S,Böhm S,Farnebo M

Sat Jun 03 00:00:00 EDT 2017

Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.

RNA biology

Coucoravas C,Dhanjal S,Henriksson S,Böhm S,Farnebo M

Sat Jun 03 00:00:00 EDT 2017

Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.

RNA biology

Coucoravas C,Dhanjal S,Henriksson S,Böhm S,Farnebo M

Sat Jun 03 00:00:00 EDT 2017

Overexpression of WRAP53 is associated with development and progression of esophageal squamous cell carcinoma.

PloS one

Rao X,Huang D,Sui X,Liu G,Song X,Xie J,Huang D

Thu Jan 14 00:00:00 EST 2016

Overexpression of WRAP53 is associated with development and progression of esophageal squamous cell carcinoma.

PloS one

Rao X,Huang D,Sui X,Liu G,Song X,Xie J,Huang D

Thu Jan 14 00:00:00 EST 2016

Overexpression of WRAP53 is associated with development and progression of esophageal squamous cell carcinoma.

PloS one

Rao X,Huang D,Sui X,Liu G,Song X,Xie J,Huang D

Thu Jan 14 00:00:00 EST 2016

Overexpression of WRAP53 is associated with development and progression of esophageal squamous cell carcinoma.

PloS one

Rao X,Huang D,Sui X,Liu G,Song X,Xie J,Huang D

Thu Jan 14 00:00:00 EST 2016

The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair.

Genes & development

Henriksson S,Rassoolzadeh H,Hedström E,Coucoravas C,Julner A,Goldstein M,Imreh G,Zhivotovsky B,Kastan MB,Helleday T,Farnebo M

Mon Dec 15 00:00:00 EST 2014

The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair.

Genes & development

Henriksson S,Rassoolzadeh H,Hedström E,Coucoravas C,Julner A,Goldstein M,Imreh G,Zhivotovsky B,Kastan MB,Helleday T,Farnebo M

Mon Dec 15 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Telomerase Cajal body protein 1; WD repeat containing, antisense to TP53; WD repeat domain 79; WD repeat-containing protein 79; WD repeat-containing protein WRAP53; WD40 protein Wrap53; WD40 repeat-containing protein antisense to TP53; WD40 repeat-containing protein antisense to TP53 gene; WD40 repeat-containing protein antisense to TP53 gene homolog; WD40 repeat-containing protein encoded by RNA antisense to p53; WD40 repeat-containing protein encoding RNA antisense to p53; WRAP53beta

Gene Aliases: BC021790; DKCB3; TCAB1; WDR79; WRAP53

UniProt ID: (Human) Q9BUR4, (Rat) Q5XII5, (Mouse) Q8VC51

Entrez Gene ID: (Human) 55135, (Rat) 287432, (Mouse) 216853

Function(s)

telomere formation via telomerase positive regulation of telomerase activity telomere maintenance via telomerase positive regulation of telomere maintenance via telomerase establishment of protein localization to telomere scaRNA localization to Cajal body telomerase RNA localization to Cajal body positive regulation of establishment of protein localization to telomere protein localization to Cajal body

Process(es)

RNA binding protein binding protein complex binding chaperone binding telomerase RNA binding