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Invitrogen
WT1 Polyclonal Antibody
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Product Details
PA5-85096
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Wilms Tumor 1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.09 mg/mL
Amount
109 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (97%), Rat (97%), Japanese Medaka (83%), Xenopus laevis (87%), Pig (97%), Chicken (94%).
Positive Control: Mouse testis, Rat testis.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins (Wilms Tumor 1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ewing sarcoma-Wilms tumor gene fusion; unnamed protein product; Wilms tumor 1; Wilms tumor protein; Wilms tumor protein homolog; Wilms' tumor protein; WT; WT33
Gene Aliases: AWT1; D630046I19Rik; GUD; NPHS4; WAGR; WIT-2; WT-1; WT1; WT33
UniProt ID: (Human) P19544, (Rat) P49952
Entrez Gene ID: (Human) 7490, (Mouse) 22431, (Rat) 24883
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
RNA binding
protein binding
zinc ion binding
double-stranded methylated DNA binding
sequence-specific DNA binding
hemi-methylated DNA-binding
metal ion binding
C2H2 zinc finger domain binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
nucleic acid binding
double-stranded DNA binding
transcription regulatory region DNA binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
vasculogenesis
ureteric bud development
branching involved in ureteric bud morphogenesis
kidney development
regulation of organ formation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
germ cell development
thorax and anterior abdomen determination
heart development
sex determination
negative regulation of cell proliferation
RNA splicing
gonad development
male gonad development
tissue development
positive regulation of gene expression
negative regulation of translation
negative regulation of cell growth
adrenal gland development
male genitalia development
epithelial cell differentiation
glomerulus development
glomerular basement membrane development
adrenal cortex formation
camera-type eye development
positive regulation of apoptotic process
negative regulation of apoptotic process
hypermethylation of CpG island
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
mesenchymal to epithelial transition
positive regulation of heart growth
diaphragm development
cardiac muscle cell fate commitment
visceral serous pericardium development
cellular response to cAMP
cellular response to gonadotropin stimulus
metanephric mesenchyme development
nephron tubule morphogenesis
glomerular visceral epithelial cell differentiation
posterior mesonephric tubule development
metanephric epithelium development
metanephric S-shaped body morphogenesis
negative regulation of metanephric glomerular mesangial cell proliferation
circulatory system development
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of male gonad development
negative regulation of female gonad development
positive regulation of metanephric ureteric bud development
metanephros development
mesonephros development
transcription, DNA-templated
transcription from RNA polymerase II promoter
apoptotic process
spermatogenesis
single fertilization
regulation of gene expression
sperm motility
Sertoli cell development
coronary vasculature development
seminiferous tubule development
negative regulation of mesenchymal cell apoptotic process involved in metanephros development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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