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Invitrogen
WTAP Polyclonal Antibody
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FIGURE: 1 / 6
WTAP Antibody (PA5-144681) in ICC/IF
Immunocytochemistry analysis of WTAP in NIH/3T3 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with WTAP polyclonal antibody (Product # PA5-144681) with a dilution of 10 µg/mL in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Nuclear DNA was labelled in ... View More
Product Details
PA5-144681
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human WTAP aa 1-300/396.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3093526
Product Specific Information
Positive control: K562 cell lysate, Jurkat cell lysate, Hela cell lysate, HepG2 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human breast carcinoma tissue, human colon tissue, mouse small intestine tissue, rat small intestine tissue, NIH/3T3.
Predicted band size: 44 kDa
Subcellular Location: Nucleus speckle, Nucleus, nucleoplasm, Cytoplasm.
Target Information
Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and is observed in both sporadic and inherited forms. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators, and functions as a transcriptional repressor. WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing. WTAP is found in nuclear speckles, where it regulates the G2/M cell cycle transition by binding to the 3' UTR of cyclin A2, thus enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and is able to impair the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Female-lethal(2)D homolog; hFL(2)D; PNAS-132; Pre-mRNA-splicing regulator WTAP; putative pre-mRNA splicing regulator female-lethal(2D); Wilms tumor 1-associating protein; Wilms' tumour 1-associating protein; WT1-associated protein
Gene Aliases: 2810408K05Rik; 9430038B09Rik; KIAA0105; Mum2; RGD1563824; WTAP
UniProt ID: (Human) Q15007, (Mouse) Q9ER69
Entrez Gene ID: (Human) 9589, (Rat) 499020, (Mouse) 60532
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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