Invitrogen

WWTR1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details

View all (16) WWTR1 antibodies

Datasheet

Datasheet

Product Image Gallery

WWTR1 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of WWTR1 in human cell line U-251 MG using a WWTR1 Polyclonal Antibody (Product # PA5-52431) shows localization to nucleoplasm, nuclear bodies and cytosol.

WWTR1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of WWTR1 in human kidney using a WWTR1 Polyclonal Antibody (Product # PA5-52431) shows moderate nuclear positivity in glomerular cells and a subset of cells in distal tubules.

WWTR1 Antibody in Western Blot (WB) — Western blot analysis of WWTR1 in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a WWTR1 Polyclonal Antibody (Product # PA5-52431). Remaining relative intensity is presented. Loading control: Anti-PPIB.

WWTR1 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of WWTR1 demonstrates antibody specificity. Immunohistochemical analysis of WWTR1 using anti-WWTR1 Polyclonal Antibody (Product # PA5-52431), shows significant staining of WWTR1 in human placenta and shows minimal or weak staining in human skeletal muscle tissues. The relative expression levels of WWTR1 within each tissue is shown using RNA-Seq. {RE}

WWTR1 Antibody in KnockDown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. EFO-21 cells were transfected with, control siRNA, WWTR1 siRNA probe #1, and WWTR1 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-WWTR1 Polyclonal Antibody (Product # PA5-52431) used at a dilution of 0.4 µg/ml. Anti-PPIB was used as a loading control. {KD}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1-4 µg/mL

Immunofluorescence (IF)

1-4 µg/mL

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human WWTR1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2649675

Product Specific Information

Immunogen sequence: MNPKPSSWRK KILPESFFKE PDSGSHSRQS STDSSGGHPG PRLAGGAQHV RSHSSPASLQ LGTGAGAAGS PAQQHAHLRQ QSYDVTDELP LPPGWEMTFT ATGQRYFLNH IEKITTWQDP RKAMNQPLNH MNLHPAVSST

Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 96%.

Target Information

Highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. A novel transcriptional co-activator, TAZ (transcriptional co-activator with PDZ-binding motif) functions as a 14-3-3-binding molecule. TAZ may link events at the plasma membrane and cytoskeleton to nuclear transcription in a manner that can be regulated by 14-3-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp586I1419; FLJ27004; FLJ45718; TAZ; transcriptional co-activator with PDZ-binding motif; transcriptional coactivator with PDZ binding motif; Transcriptional coactivator with PDZ-binding motif; WW domain-containing transcription regulator protein 1

Gene Aliases: 2310058J06Rik; 2610021I22Rik; C78399; TAZ; WWTR1

UniProt ID: (Human) Q9GZV5, (Mouse) Q9EPK5

Entrez Gene ID: (Human) 25937, (Mouse) 97064

Molecular Function: enzyme modulator kinase modulator transcription cofactor transcription factor