Invitrogen

WWTR1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (19) WWTR1 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

WWTR1 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of WWTR1 in human cell line U-251 MG using a WWTR1 Polyclonal Antibody (Product # PA5-52431) shows localization to nucleoplasm, nuclear bodies and cytosol.

WWTR1 Antibody in Immunocytochemistry (ICC) — Immunofluorescence analysis of WWTR1 was performed using 70% confluent log phase HeLa and HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with WWTR1 Polyclonal Antibody (Product # PA5-52431) at 1 µg/ml in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HeLa cells, which is a positive model for WWTR1 expression showing a nuclear and cytoplasmic localization. Panel e represents the merged image of HT-29 cells, that are null for WWTR1 protein expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

WWTR1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of WWTR1 in human kidney using a WWTR1 Polyclonal Antibody (Product # PA5-52431) shows moderate nuclear positivity in glomerular cells and a subset of cells in distal tubules.

WWTR1 Antibody in Western Blot (WB) — Western blot was performed using Anti-WWTR1 Polyclonal Antibody (Product # PA5-52431) and a 55kDa band corresponding to WWTR1 was observed across cell lines tested except HT-29, K-562 and MOLT-4. This product does not show any reactivity to Mouse and Rat tissues. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), Hep G2 (Lane 3), HCT 116 (Lane 4), HT-29 (Lane 5), K-562 (Lane 6), MOLT-4 (Lane 7), Mouse Heart (Lane 8), Rat Heart (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

WWTR1 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. EFO-21 cells were transfected with, control siRNA, WWTR1 siRNA probe #1, and WWTR1 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-WWTR1 Polyclonal Antibody (Product # PA5-52431) used at a dilution of 0.4 µg/ml. Anti-PPIB was used as a loading control. {KD}

WWTR1 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of WWTR1 was observed in HeLa (Lane 1), A549 (Lane 2), Hep G2 (Lane 3), HCT 116 (Lane 4) cell lines that express WWTR1, in comparison to HT-29 (Lane 5), K-562 (Lane 6) and MOLT-4 (Lane 7) cell lines that are null for WWTR1 expression, using Anti-WWTR1 Polyclonal Antibody (Product # PA5-52431) in Western Blot. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

0.25-2 µg/mL

Immunofluorescence (IF)

0.25-2 µg/mL

Immunohistochemistry (IHC)

1:200-1:500

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species

Human, Mouse, Rat

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human WWTR1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2649675

Product Specific Information

Immunogen sequence: MNPKPSSWRK KILPESFFKE PDSGSHSRQS STDSSGGHPG PRLAGGAQHV RSHSSPASLQ LGTGAGAAGS PAQQHAHLRQ QSYDVTDELP LPPGWEMTFT ATGQRYFLNH IEKITTWQDP RKAMNQPLNH MNLHPAVSST

Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 96%.

Target Information

WW domain-containing transcription regulator protein 1 (WWTR1) is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor supression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1. In turn, MOB1 phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. WWTR1 regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. WWTR1 also regulates embryonic stem-cell self-renewal and promotes cell proliferation and epithelial-mesenchymal transition.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this antibody

Bioinformatics

Protein Aliases: DKFZp586I1419; FLJ27004; FLJ45718; transcriptional co-activator with PDZ-binding motif; transcriptional co-activator with PDZ-binding motif (TA)Z; transcriptional co-activator with PDZ-binding motif (TAZ); transcriptional coactivator with PDZ binding motif; Transcriptional coactivator with PDZ-binding motif; WW domain-containing transcription regulator protein 1

Gene Aliases: 2310058J06Rik; 2610021I22Rik; C78399; TAZ; WWTR1

UniProt ID: (Human) Q9GZV5, (Mouse) Q9EPK5

Entrez Gene ID: (Human) 25937, (Mouse) 97064, (Rat) 295062

Molecular Function: enzyme modulator kinase modulator transcription cofactor transcription factor