Invitrogen

WWTR1 Polyclonal Antibody

Product Image Gallery

WWTR1 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of WWTR1 in human cell line U-251 MG using a WWTR1 Polyclonal Antibody (Product # PA5-52431) shows localization to nucleoplasm, nuclear bodies and cytosol.

WWTR1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of WWTR1 in human renal cancer using a WWTR1 Polyclonal Antibody (Product # PA5-52431) shows strong nuclear positivity in tumor cells.

WWTR1 Antibody in Western Blot (WB) — Western blot analysis of WWTR1 in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a WWTR1 Polyclonal Antibody (Product # PA5-52431). Remaining relative intensity is presented. Loading control: Anti-PPIB.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1-4 µg/mL

Immunofluorescence (IF)

1-4 µg/mL

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human WWTR1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2649675

Product Specific Information

Immunogen sequence: MNPKPSSWRK KILPESFFKE PDSGSHSRQS STDSSGGHPG PRLAGGAQHV RSHSSPASLQ LGTGAGAAGS PAQQHAHLRQ QSYDVTDELP LPPGWEMTFT ATGQRYFLNH IEKITTWQDP RKAMNQPLNH MNLHPAVSST

Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 96%.

Target Information

Highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. A novel transcriptional co-activator, TAZ (transcriptional co-activator with PDZ-binding motif) functions as a 14-3-3-binding molecule. TAZ may link events at the plasma membrane and cytoskeleton to nuclear transcription in a manner that can be regulated by 14-3-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp586I1419; FLJ27004; FLJ45718; TAZ; transcriptional co-activator with PDZ-binding motif; transcriptional coactivator with PDZ binding motif; Transcriptional coactivator with PDZ-binding motif; WW domain-containing transcription regulator protein 1

Gene Aliases: 2310058J06Rik; 2610021I22Rik; C78399; TAZ; WWTR1

UniProt ID: (Human) Q9GZV5, (Mouse) Q9EPK5

Entrez Gene ID: (Human) 25937, (Mouse) 97064

Molecular Function: enzyme modulator kinase modulator transcription cofactor transcription factor