Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
WWTR1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 15
WWTR1 Antibody (PA5-52431) in ICC/IF
Immunofluorescence analysis of WWTR1 was performed using 70% confluent log phase HeLa and HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with WWTR1 Polyclonal Antibody (Product # PA5-52431) at 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A... View More
Product Details
PA5-52431
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human WWTR1. Recombinant protein control fragment (Product #RP-89285).
Conjugate
Form
Liquid
Concentration
0.10 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2649675
Product Specific Information
Immunogen sequence: MNPKPSSWRK KILPESFFKE PDSGSHSRQS STDSSGGHPG PRLAGGAQHV RSHSSPASLQ LGTGAGAAGS PAQQHAHLRQ QSYDVTDELP LPPGWEMTFT ATGQRYFLNH IEKITTWQDP RKAMNQPLNH MNLHPAVSST
Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 96%.
Target Information
WW domain-containing transcription regulator protein 1 (WWTR1) is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor supression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1. In turn, MOB1 phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. WWTR1 regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. WWTR1 also regulates embryonic stem-cell self-renewal and promotes cell proliferation and epithelial-mesenchymal transition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DKFZp586I1419; FLJ27004; FLJ45718; Tafazzin; transcriptional co-activator with PDZ-binding motif; transcriptional coactivator with PDZ binding motif; Transcriptional coactivator with PDZ-binding motif; WW domain-containing transcription regulator protein 1
Gene Aliases: 2310058J06Rik; 2610021I22Rik; C78399; TAZ; WWTR1
UniProt ID: (Human) Q9GZV5, (Mouse) Q9EPK5
Entrez Gene ID: (Human) 25937, (Mouse) 97064
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
negative regulation of protein phosphorylation
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
negative regulation of protein kinase activity
positive regulation of cell proliferation
positive regulation of epithelial to mesenchymal transition
protein ubiquitination
stem cell division
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
glomerulus development
multicellular organism growth
hippo signaling
negative regulation of catenin import into nucleus
negative regulation of fat cell differentiation
positive regulation of transcription from RNA polymerase II promoter
mesenchymal cell differentiation
cilium morphogenesis
regulation of SMAD protein import into nucleus
kidney morphogenesis
regulation of metanephric nephron tubule epithelial cell differentiation
negative regulation of canonical Wnt signaling pathway
transcription, DNA-templated
regulation of canonical Wnt signaling pathway
regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support