The Wnt surrogate transcriptome is highly similar to Wnt3a-regulated transcriptomes. Untreated iPSCs were cultured in DMEM/F-12 in the absence of Wnt agonist; StemFlex Medium is the PSC culture medium. All conditions were assayed 3 times as indicated by the labels on the left of the heat map. The heat map contains 4,744 genes with log2(rpm+1) and > 2.5-fold change in gene expression threshold; rpm = reads p... View More

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Product Details

PHG0401

Applications

Tested Dilution

Publications

Bioactivity (Bioactivity)

Assay-dependent

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Product Specifications

Species

Human

Published species

Human

Expression System

HEK293 cells

Class

Recombinant

Type

Protein

Purity

> 95%

Endotoxin concentration

1 EU/mL

Activity

Greater than 25 fold induction in Topflash assay activity at a Wnt surrogate-Fc protein concentration between 0.03 - 5.0 nM (based on dimeric stoichiometry)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Amount

25 µg

Purification

purified

Storage buffer

25mM tris, pH 8.2, with 500mM NaCl

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Dry ice

Product Specific Information

Gibco Wnt Surrogate-Fc Fusion Protein activates similar Wnt signaling pathways as most commercially available recombinant Wnt3a proteins or Wnt-conditioned media, while requiring a fraction of the amount of protein or hassle. Gibco Wnt Surrogate-Fc Fusion Protein was engineered with improved solubility, overcoming a major obstacle of working with Wnt3a and reducing the concentrations required to activate Wnt signaling in your model system.

Gibco Wnt Surrogate-Fc Fusion Protein is comprised of the LRP-binding domain of DKK linked to a selective, high affinity binder of Frizzled receptors.

Gibco Wnt Surrogate-Fc Fusion Protein is provided at a concentration of 2 mg/mL which is equivalent to 17.1 µM based on dimeric stoichiometry.

Storage: Store undiluted Gibco Wnt Surrogate-Fc Fusion Protein at -80° for up to 1 year. After thawing, apportion into working aliquots and store at -20°C to -80°C for up to 2 months.

Target Information

Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: MGC119418; MGC119419; MGC119420; Protein Wnt-3a; unnamed protein product; wingless-type MMTV integration site family, member 3A; Wnt 3a; WNT3A protein

Wnt Surrogate-Fc Fusion Recombinant Protein

Wnt Surrogate-Fc Fusion Protein (PHG0401) in Bioactivity