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Wnt10B Antibody (PA5-116125) in ICC/IF
Immunocytochemistry analysis of Wnt10B in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116125) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-116125
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human WNT10B(Accession O00744), corresponding to amino acid residues R169-A219.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900759
Product Specific Information
Antibody detects endogenous levels of total Wnt10b.
Target Information
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein Wnt-10b; Protein Wnt-12; wingless related MMTV integration site 10b; wingless-type MMTV integration site family member 10B; wingless-type MMTV integration site family, member 10B; WNT-10B protein; wnt-12
Gene Aliases: SHFM6; Wnt-10b; WNT-12; Wnt10; WNT10B; WNT12
UniProt ID: (Human) O00744, (Mouse) P48614
Entrez Gene ID: (Human) 7480, (Mouse) 22410
G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
chondrocyte differentiation
lipid metabolic process
cell cycle arrest
smoothened signaling pathway
positive regulation of cell proliferation
positive regulation of G2/M transition of mitotic cell cycle
myoblast differentiation involved in skeletal muscle regeneration
Wnt signaling pathway
neuron differentiation
positive regulation of bone mineralization
positive regulation of epithelial cell differentiation
regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of apoptotic process
cell fate commitment
negative regulation of fat cell differentiation
positive regulation of osteoblast differentiation
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly
regulation of skeletal muscle tissue development
skeletal muscle fiber development
negative regulation of epithelial cell proliferation
protein stabilization
sensory perception of taste
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of anagen
canonical Wnt signaling pathway
bone trabecula formation
fungiform papilla development
cellular response to retinoic acid
cellular response to cAMP
cellular response to parathyroid hormone stimulus
hematopoietic stem cell proliferation
cellular response to hydrostatic pressure
positive regulation of canonical Wnt signaling pathway
regulation of transcription from RNA polymerase II promoter
signal transduction
cell-cell signaling
multicellular organism development
organ morphogenesis
skeletal muscle tissue regeneration
regulation of fat cell differentiation
positive regulation of ossification
positive regulation of transcription from RNA polymerase II promoter
regulation of protein metabolic process
cellular response to organic substance
cellular response to organic cyclic compound
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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