Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:200-1:1,000 | View 6 publications 6 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:500 | - |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Miscellaneous PubMed (Misc) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
XBP1 Fusion Protein Ag21714 (167-261 aa encoded by BC000938) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.41 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: LRLRAPLQQ VQAQLSPLQN ISPWILAVLT LQIQSLISCW AFWTTWTQSC SSNALPQSLP AWRSSQRSTQ KDPVPYQPPF LCQWGRHQPS WKPLMN (167-261 aa encoded by BC000938)
XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Hepatocarcinogenesis-related transcription factor; HTF; OTTHUMP00000028682; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB-5; X-box-binding protein 1; X-box-binding protein 1, cytoplasmic form; X-box-binding protein 1, luminal form; XBP-1
Gene Aliases: D11Ertd39e; HTF; TREB-5; TREB5; XBP-1; XBP1; XBP2
UniProt ID: (Human) P17861, (Rat) Q9R1S4, (Mouse) O35426
Entrez Gene ID: (Human) 7494, (Rat) 289754, (Mouse) 22433
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