Proteintech

XBP1 Polyclonal Antibody

XBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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XBP1 Antibody (25997-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 25997-1-AP ( XBP1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

25997-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 40 publications 40 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

XBP1 Fusion Protein Ag21714 (167-261 aa encoded by BC000938)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LRLRAPLQQ VQAQLSPLQN ISPWILAVLT LQIQSLISCW AFWTTWTQSC SSNALPQSLP AWRSSQRSTQ KDPVPYQPPF LCQWGRHQPS WKPLMN (167-261 aa encoded by BC000938)

Target Information

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Identification of XBP1-u as a novel regulator of the MDM2/p53 axis using an shRNA library.

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Identification of XBP1-u as a novel regulator of the MDM2/p53 axis using an shRNA library.

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Hepatocyte X-box binding protein 1 deficiency increases liver injury in mice fed a high-fat/sugar diet.

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Bioinformatics

Protein Aliases: Hepatocarcinogenesis-related transcription factor; HTF; OTTHUMP00000028682; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB-5; X-box-binding protein 1; XBP-1

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Gene Aliases: D11Ertd39e; HTF; TREB-5; TREB5; XBP-1; XBP1; XBP2

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UniProt ID: (Human) P17861, (Rat) Q9R1S4, (Mouse) O35426

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Entrez Gene ID: (Human) 7494, (Rat) 289754, (Mouse) 22433

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding phosphatidylinositol 3-kinase regulatory subunit binding histone deacetylase binding protein heterodimerization activity RNA polymerase II regulatory region sequence-specific DNA binding protease binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding sequence-specific DNA binding transcription regulatory region DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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XBP1 Polyclonal Antibody

XBP1 Antibody (25997-1-AP) in IHC (P)

Product Details

25997-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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